Thermo Scientific™ Pierce™ GTPase Enrichment Kit and Probes
A desthiobiotin-GTP probe kit to label, affinity purify and measure the active site of GTPases and the GTPase subunits of G-protein coupled receptors.
Manufacturer: Thermo Scientific™ 88315
Thermo Scientific Pierce GTPase Enrichment Kits use ActivX™ GTP Probes to covalently label the active site of GTPases and the GTPase subunits of G-protein coupled receptors enabling their selective enrichment using the desthiobiotin tag.
ActivX GTP Probes feature a desthiobiotin (biotin analog) tag that facilitates selective enrichment and recovery of labeled GTPases allowing identification and profiling of target enzyme classes across samples or to assess the specificity and affinity of enzyme inhibitors.
Specific – label only the conserved active-site lysines of nucleotide-binding proteins
Compatible – use for in vitro labeling of GTPase enzymes derived from cells or tissues.
Flexible – use with Western blot or mass spectrometry (MS) workflows
Broad enrichment of GTP-binding proteins from tissues, cells and subcellular proteomes; Enrichment of enzymes based on function; Profiling of dozens to hundreds of inhibitor targets.
|ActivX Desthiobiotin-GTP Probe|
|16 pull-down assays|
|Desthiobiotin-GTP, MW 1275|
|16 x 12.9µg|
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