missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. PI21516

Thermo Scientific™ Pierce™ GST Protein Interaction Pull-Down Kit

Catalog No. PI21516
Change view
Click to view available options
Quantity:
25 reactions kit
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PI21516 25 reactions kit
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PI21516 Supplier Thermo Scientific™ Supplier No. LSG21516
Only null left

Description

Easily capture and affinity purify binding partners of GST-tagged fusion proteins with these glutathione (GSH) agarose beads and complete reagent set.

Thermo Fisher Scientific Pierce GST Tag Protein Interaction Pull-Down Kits contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.

Features of GST Tag Protein Interaction Pull-Down Kit:

  • GST pull-down (Product No. 21516)—gently purifies protein interactors of any GST-tagged fusion protein without denaturing
  • Complete kits—provide all components and detailed protocol for purifying protein:protein interactions
  • No special equipment needed—use common laboratory equipment and reagents (e.g., microcentrifuge)
  • Convenient—microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
  • Flexible—instructions include protocols for use with bait and prey proteins from many different sources
  • Binding—Binds approx. 8 mg of GST-tagged fusion protein per mL of resin

Applications:

  • Discover a new protein:protein interaction from a cell lysate
  • Confirm a putative interaction from a cell lysate or with a previously purified protein
  • Extract protein:protein interaction information from in vitro transcription/translation lysates

You provide the tagged fusion protein as the 'bait' and the cells expressing the putative protein interaction target ('prey'), and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.

Related Products

Pierce™ His Protein Interaction Pull-Down Kit (Catalog #21277)

Specifications

Bed Volume 750 μL
Capacity (Metric) 1.5 mL
Formulation Slurry: 50 %
Content And Storage Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin
• Glutathione Agarose, 750 μL
• Pull-Down Lysis Buffer, 250 mL
• Glutathione, 1 g
• TBS Pack (makes 500 mL), 1 pack
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 100 tubes

Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.
Assay Affinity Purification
For Use With (Equipment) Microcentrifuge
Product Line Pierce
Quantity 25 reactions kit
For Use With (Application) Pull-Down (Tag-Based)
Target Not Target-Specific
Type Pull-Down Kit
Sufficient For 25 Reactions
Format Kit
Show More Show Less

Frequently Asked Questions (FAQs)

If my protein is endogenously expressed, which Thermo Scientific Protein:Protein Interaction (PPI) kit should I choose?

We recommend the biotin kit for endogenously expressed proteins. The reason for this is the wide variety of biotinylation reagents that can be used to label the detection protein of the binding pair. For help choosing a biotinylation reagent, refer to the Biotinylation Selection Guide available at our website.
How is the tagged protein detected on the membrane using the Thermo Scientific Protein:Protein Interaction (PPI) kits?

The biotin kit utilizes streptavidin-HRP to detect the biotinylated protein bound to the prey protein on the membrane. The GST kit utilizes a polyclonal goat anti-GST antibody for detection of the glutathione-S-transferase tagged fusion protein bound to another protein on a membrane.
How many reactions can be performed using one Thermo Scientific Protein:Protein Interaction (PPI) kit?

The Thermo Scientific Pull-Down Protein:Protein Interaction Kits contain a complete, validated set of reagents specifically developed for performing pull-down assays.The kits contain sufficient materials for conducting 25 pull-down assays.
Are all of the conditions for binding the prey protein to the bait protein described in the Thermo Scientific Protein:Protein Interaction (PPI) kits?

No. Every protein pair will require unique conditions in order to bind with one another. Factors to consider are pH, ionic strength of the buffer, need for cofactors, whether the interaction is long term or transient, etc. The researcher is recommended to perform a literature search or try several different conditions in order to determine what conditions are best for the binding of their proteins of interest. The kit includes a “universal“ wash buffer formed by diluting the lysis buffer 1:1 in TBS.
With the Thermo Scientific Protein:Protein Interaction (PPI) kits, what methods can be used to detect the prey protein?

The prey protein can be detected following transfer on a membrane. The membrane procedures uses 1:100,000 - 1:500,000 dilution of the anti-GST antibody.
What types of Thermo Scientific Protein:Protein Interaction (PPI) kits are available?

There are Pull-Down Kits and Far-Western Kits. The Pull-Down Kits are very similar to an immunoprecipitation (IP), but instead of an antibody, a tagged bait protein [biotin (Cat. No. 21115) , GST (Cat. No. 21516) or 6xHis (Cat. No. 21277)] is used to bind the prey or target protein. This protein complex is then purified using an affinity column specific for the tagged bait protein. The Far Western Kits are similar to Western Blots, but again, instead of an antibody, a tagged non-immunoglobulin protein [biotin (Cat. No. 23500) or GST (Cat. No. 23505)] is used to bind to the prey or target protein for detection on the membrane.

For Research Use Only. Not for use in diagnostic procedures.