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Thermo Scientific™ Pierce™ Glutathione Agarose Resin and Kits

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

$93.71 - $1,799.70

Products
Catalog Number Mfr. No. Description Quantity Price Quantity    

PI16100

 
thermo scientific™
16100
Pierce Glutathione Agarose 10mL Each for $337.05

PI16101

 
thermo scientific™
16101*
Pierce Glutathione Agarose 100mL Each for $1,799.70

PI16103

 
thermo scientific™
16103
Pierce Glutathione Spin Columns, 0.2mL 25 columns Pack of 25 Each for $317.10

PI16104

 
thermo scientific™
16104
Pierce Glutathione Spin Columns, 1mL 5 columns Pack of 5 Each for $317.10

PI16105

 
thermo scientific™
16105*
Pierce Glutathione Spin Columns, 3mL 5 columns Pack of 5 Each for $569.10

PI16106

 
thermo scientific™
16106
Pierce GST Spin Purification Kit, 0.2mL 25 column kit Each for $494.55

PI16107

 
thermo scientific™
16107
Pierce GST Spin Purification Kit, 1mL 5 column kit Each for $494.55

PI16108

 
thermo scientific™
16108
Pierce GST Spin Purification Kit, 3mL 5 column kit Each for $680.40

PI16109

 
thermo scientific™
16109
Pierce Glutathione Chromatography Cartridges, 1mL 5 cartridges Pack of 5 Each for $413.70

PI16110

 
thermo scientific™
16110
Pierce Glutathione Chromatography Cartridges, 5mL 2 cartridges Pack of 2 Each for $732.90

PI78259

 
thermo scientific™
78259
Pierce Glutathione 5 x 184mg Pack of 5 Each for $93.71

PI16111

 
thermo scientific™
16111*
Pierce Glutathione Spin Plates 2 plates Each for $779.10
Description & Specifications

Thermo Scientific™ Pierce Glutathione Agarose is a high-capacity, high-performance resin for affinity purification of GST-tagged fusion proteins from cellular lysates.

The high–quality support consists of glutathione that has been immobilized by its central sulfhydryl group via a 12–atom spacer arm to crosslinked 6% beaded agarose. Purification of GST–fusion proteins using glutathione (GSH) agarose beads is well documented and adaptable to a variety of scales, column formats and specific applications. Whether the purpose is to purify large amounts of recombinant protein from over–expressing E. Coli lysates or to investigate protein interactions involving GST–tagged bait proteins, Pierce Glutathione Agarose is suitable for the task.

To accommodate these many uses, Pierce Glutathione Agarose is offered in several package sizes and formats. These include three volumes of resin slurry, three sizes of centrifuge-ready columns, complete GST purification kits, and two sizes of FPLC–ready chromatography cartridges.

Highlights:

  • High capacity – binds at least 25mg of recombinant GST protein per milliliter of resin

  • High yield and purity – consistently purifies at least 10mg of GST – tagged protein per milliliter of resin with greater than 90% purity

  • Cost-effective – resin is economically priced and can be reused at least five times without reduction in binding capacity and purification performance

  • Versatile – works well to purify GST – fusion proteins from bacterial lysates or use with pre-purified GST – tagged proteins to pull down protein interactions

  • Compatible – validated and effective for use with Thermo Scientific Pierce Cell Lysis Reagents to extract and purify from bacterial or mammalian cell cultures

  • Flexible – available in multiple formats including bulk resin, spin columns, chromatography cartridges and convenient GST purification kits

Available Formats:

  • Resin Slurries: 10mL, 100mL, 500mL bottles

  • Spin Columns: 0.2mL (microcentrifuge), 1mL, 3mL columns

  • Purification Kits: complete kits in all three column sizes

  • Chromatography Cartridges: 1mL and 5mL cartridges, compatible with automated liquid chromatography systems and with syringe processing

  • Bulk and Custom: let us know your needs for bulk Glutathione Agarose

Includes:

Kits contain Pierce Glutathione Spin Columns, equilibration/wash buffer and glutathione to prepare elution buffer