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Thermo Scientific™ EGS (ethylene glycol bis(succinimidyl succinate))

Catalog No. pi21565
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PI21565 1 g
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Catalog No. PI21565 Supplier Thermo Scientific™ Supplier No. LSG21565
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Description

Crosslink amines to amines with this membrane permeable crosslinker composed of NHS-ester groups and a 16.1A hydroxylamine-cleavable spacer arm.

Thermo Scientific™ Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.

Highlights:

  • Cleavable at pH 8.5 using Hydroxylamine for 3-6 hours at 37°C
  • Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS
  • Reactive groups: NHS esters (homobifunctional)
  • Reactive toward: amino groups

Specifications

Chemical Reactivity Amine-Amine
Cleavable By Hydroxylamine
Description EGS
Molecular Weight (g/mol) 456.36
PEGylated No
Spacer Arm Length 16.1 Å
Content And Storage Upon receipt store at 4°C.
Cell Permeability Yes
Shipping Condition Ambient
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Homobifunctional
Reactive Moiety NHS Ester
Spacer Medium (10 to 30 Å)
Form Powder
Quantity 1 g
Solubility DMF, DMSO
Format Standard
Water Soluble No
Product Type Crosslinker
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Frequently Asked Questions (FAQs)

Can you provide the shelf-life for EGS (ethylene glycol bis(succinimidyl succinate))?

EGS (ethylene glycol bis(succinimidyl succinate)) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
Can NHS-diazirine (Cat. No. 26167) be used for CHIP applications?

NHS-diazirine has not been tested for cross-linking in a ChIP application. EGS (Cat. No. 21565) and DSG (Cat. No. 20593) are not used by themselves in ChIP, but rather in combination with formaldehyde. While formaldehyde is good for crosslinking proteins that are in direct contact with DNA, it cannot trap proteins that may be bound in a complex with other proteins but are not in direct contact. Thus EGS or DSG can cross-link proteins to proteins that are in direct contact with DNA and are crosslinked to it by formaldehyde. See this reference for more information

For Research Use Only. Not for use in diagnostic procedures.