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Thermo Scientific™ DTSSP (3,3'-dithiobis(sulfosuccinimidyl propionate))

Catalog No. pi21578
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50 mg
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Catalog No. Quantity
PI21578 50 mg
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Catalog No. PI21578 Supplier Thermo Scientific™ Supplier No. LSG21578
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Description

Crosslink amines to amines with Sulfo-DSP; composed of sulfo-NHS-ester groups and disulfide spacer arm (12.0A) that is cleavable with reducing agents.

Thermo Scientific™ Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm and whose central disulfide bond can be cleaved with reducing agents.

Highlights:

  • Water-soluble
  • Cleavable by reducing agents
  • Reactive groups: sulfo-NHS ester (homobifunctional)
  • Reactive toward: amino groups

Specifications

Chemical Reactivity Amine-Amine
Cleavable By Thiols
Description DTSSP
Molecular Weight (g/mol) 608.51
PEGylated No
Spacer Arm Length 12.0 Å
Content And Storage Upon receipt store desiccated at 4°C.
Cell Permeability No
Shipping Condition Ambient
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Homobifunctional
Reactive Moiety Sulfo-NHS Ester
Spacer Medium (10 to 30 Å)
Form Powder
Quantity 50 mg
Solubility Water
Format Standard
Water Soluble Yes
Product Type Crosslinker
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Frequently Asked Questions (FAQs)

Can you provide the shelf-life for DTSSP (3,3'-dithiobis(sulfosuccinimidyl propionate))?

DTSSP (3,3'-dithiobis(sulfosuccinimidyl propionate)) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
What is the difference between a homo- and a hetero- bifunctional cross-linker?

A homobifunctional cross-linker has the same reactive group on both ends, for example two amine-reactive groups. It is used in a one-step conjugation where the two molecules are mixed prior to the addition of the cross-linker. This leads to less efficient cross-linking because polymers can easily be created. A heterobifunctional cross-linker has two different reactive groups, for example an amine-reactive group on one end and a sulhydryl reactive group on the other. These can be used in two-step conjugations by reacting the fIrst end with the molecule containing its reactive group, and then adding the molecule that is reactive towards the other end. This leads to much more efficient cross-linking because polymerization does not occur.

For Research Use Only. Not for use in diagnostic procedures.