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Thermo Scientific™ Pierce™ DSG, No-Weigh™ Format

Catalog No. pi20593
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50 mg
10 x 1 mg
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Catalog No. Quantity
PI20593 50 mg
PIA35392 10 x 1 mg
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Catalog No. PI20593 Supplier Thermo Scientific™ Supplier No. 20593

Description

Thermo Scientific™ Pierce DSG is disuccinimidyl glutarate, a simple homobifunctional crosslinker based on the amine-reactive N-hydroxysuccinimide (NHS) ester group.

Highlights:

  • Can increase crosslinking efficiency compared to DSS in some applications
  • Reactive groups: NHS ester (homobifunctional)
  • Reactive toward: amino groups

Specifications

Chemical Reactivity Amine-Amine
Cleavable No
Molecular Weight (g/mol) 326.26
PEGylated No
Spacer Arm Length 7.7 Å
Content And Storage Upon receipt store desiccated at 4°C.
Cell Permeability Yes
Shipping Condition Ambient
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Homobifunctional
Reactive Moiety NHS Ester
Spacer Short (<10 Å)
Form Powder
Quantity 50 mg
Solubility DMF, DMSO
Format Standard, Single-use
Water Soluble No
Product Type Crosslinker
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Frequently Asked Questions (FAQs)

Can you provide the shelf-life for DSG (disuccinimidyl glutarate)?

DSG (disuccinimidyl glutarate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
How should I dissolve and store DSG, No-Weigh Format?

DSG is an NHS-ester that hydrolyzes easily and is moisture-sensitive. For optimal results, we recommend storing DSG powder desiccated at 4 degrees C. To avoid moisture condensation onto the product, the vial must be equilibrated to room temperature before opening. We recommend resuspending DSG in DMSO, Anhydrous (Cat. No. D12345) right before use and not storing the solution.
Can NHS-diazirine (Cat. No. 26167) be used for CHIP applications?

NHS-diazirine has not been tested for cross-linking in a ChIP application. EGS (Cat. No. 21565) and DSG (Cat. No. 20593) are not used by themselves in ChIP, but rather in combination with formaldehyde. While formaldehyde is good for crosslinking proteins that are in direct contact with DNA, it cannot trap proteins that may be bound in a complex with other proteins but are not in direct contact. Thus EGS or DSG can cross-link proteins to proteins that are in direct contact with DNA and are crosslinked to it by formaldehyde. See this reference for more information

For Research Use Only. Not for use in diagnostic procedures.