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Thermo Scientific™ DNase I Solution (2500 U/mL)

Catalog No. PI90083
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PI90083 0.5 mL
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Catalog No. PI90083 Supplier Thermo Scientific™ Supplier No. 90083
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Description

Improve protein extraction efficiency with this glycosylated polypeptide that cleaves single- and double-stranded DNA in cell lysates.

Remove unwanted DNA from cell lysates to improve protein extraction efficiency with Thermo Scientific™ DNase I.

Deoxyribonuclease I (DNase I) is a single, glycosylated polypeptide that degrades unwanted single- and double-stranded DNA. The enzyme works by cleaving DNA into 5' phosphodinucleotide and small oligonucleotide fragments. DNase I is commonly added to cell lysis reagents to remove the viscosity caused by the DNA content in bacterial cell lysates or to remove the DNA templates from RNAs produced by in vitro transcription. Two grades of DNAse are offered, one sufficient for protein work and one that is useful for any application requiring the digestion of DNA in which it is crucial to avoid damage to RNA.

Highlights:

  • Degrades and removes unwanted DNA from samples
  • Cleaves both single-stranded and double-stranded DNA
  • Compatible with Thermo Scientific Pierce Cell Lysis Reagents
  • Reduces viscosity of bacterial lysates (protein extracts) to facilitate pipetting
  • Supplied as 50% glycerol stocks in 10mM Tris-HCl pH 7.5, 10mM CaCl2, 10mM MgCl2
  • Available in two convenient formats: RNase-tested to protect RNA (Part No. 89835) and convenient protein extraction grade (Part No. 90083)

Recommended for:

Elimination of viscosity caused by DNA in protein cell lysates; Removal of DNA templates from RNAs produced by in vitro transcription

Specifications

Product Type Cell Lysis Enzyme
Content And Storage Store at -20°C
Form Liquid
Enzyme DNase
Quantity 0.5 mL

Frequently Asked Questions (FAQs)

How much B-PER Reagent should I use for large-scale preps?

Using 1 L of E.coli culture with an OD600 of approximately 2 will give approximately 8 grams wet cells. For 8 g cells, use 40-50 mL of B-PER Reagent.
Can you explain how the B-PER Bacterial Protein Extraction Reagent lyses cells?

The B-PER Reagent solution contains a proprietary, mild, non-ionic detergent in 20 mM Tris-HCl, pH 7.5. It effectively disrupts cells and solubilizes native or recombinant proteins without denaturation. The reagent creates holes in the cell membrane that will leak out cytosolic proteins. The sample may become very viscous when the bacterial chromosome is released. We recommend adding DNAse I (Cat. No. 90083) to the reagent to reduce viscosity. For better lysis efficiency and if there are inclusion bodies, we recommend adding Lysozyme (Cat. No. 90082) to the reagent. Alternatively, you may purchase the B-PER Bacterial Protein Extraction Reagent with Enzymes Kit (Cat. No. 90078 or 90079) that includes the B-PER Bacterial Protein Extraction Reagent, DNase I, and Lysozyme.

For Research Use Only. Not for use in diagnostic procedures.