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Thermo Scientific™ Pierce™ Controlled Protein-Protein Crosslinking Kit

Catalog No. PI23456
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PI23456 1 Reaction
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Catalog No. PI23456 Supplier Thermo Scientific™ Supplier No. LSG23456
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Description

Chemically conjugate any two purified proteins (antibodies, enzymes, carriers, etc.) using sulfo-SMCC crosslinker, a complete kit with modification reagents and buffers.

Thermo Scientific™ Pierce Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.

The kit provides Sulfo-SMCC crosslinker for amine-to-sulfhydryl conjugation and SATA modification reagent for specific sulfhydryl-addition. The crosslinking strategy allows two purified proteins, such as an antibody and a reporter enzyme, to be conjugated in a two-step, controlled manner. Supplementary components, buffers and columns are also included to support successful creation of a useful conjugate via any one of several specific protocols.

Highlights:

  • Sulfo-SMCC – high-quality crosslinker conjugates primary amines of protein 1 to sulfhydryl groups of protein 2

  • SATA or 2-MEA – modification reagents provide two options for making sulfhydryl groups available on protein 2 (usually an antibody)

  • Ellman's Reagent – provides a mean of monitoring and assessing efficiency of the final reaction

  • Complete – includes critical reagents as well as buffers, desalting columns and supplementary modification reagents

Specifications

Chemical Reactivity Amine-Sulfhydryl
Cleavable No
Description Controlled Protein-Protein Crosslinking Kit
Molecular Weight (g/mol) 436.37
PEGylated No
Spacer Arm Length 11.6 Å
Content And Storage Sufficient For: One conjugation with two purified proteins (up to 5 mg each)
• Sulfo-SMCC Crosslinking Agent, 2 mg
• Activation/Conjugation Buffer (10X), 20 mL
• PBS packs (each makes 500mL), 2 packs
• 2-Mercaptoethylamine-HCl, 6 mg
• Immobilized-Reductant, 0.2 mL
• SATA, 2 mg
• Dimethylformamide (DMF), 1 mL
• Hydroxylamine-HCl, 5 mg
• Dextran Desalting Column, 2 x 10 mL
• Column Extender, 2 ea.
• Cysteine-HCl, 20 mg
• Ellman's Reagent, 2 mg

Store at 4°C.
Cell Permeability No
Shipping Condition Ambient
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety Maleimide, Sulfo-NHS Ester
Spacer Short (<10 Å)
Form Kit
Quantity 1 Reaction
Solubility Water
Format Kit
Water Soluble Yes
Product Type Crosslinker
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For Research Use Only. Not for use in diagnostic procedures.