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Thermo Scientific™ Pierce™ ChIP-grade Protein A/G Magnetic Beads

Catalog No. PI26162
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PI26162 5 mL
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Catalog No. PI26162 Supplier Thermo Scientific™ Supplier No. LSG26162

Description

Efficiently isolate chromatin-bound DNA by immunoprecipitation (chromatin IP) for subsequent quantitation by PCR using Thermo Scientific™ Pierce Magnetic ChIP Kit.

Efficiently isolate chromatin-bound DNA by immunoprecipitation (chromatin IP) for subsequent quantitation by PCR using Thermo Scientific™ Pierce Magnetic ChIP Kit.

Additional Information and Online Ordering

The Pierce Magnetic ChIP Kit contains sufficient reagents to perform 30 chromatin immunoprecipitation (ChIP) assays with appropriate controls using an optimized protocol. The kit includes reagents for cell lysis, capture of protein-DNA complexes, reversal of crosslinking, and DNA isolation. The blocked Pierce Protein A/G Magnetic Beads provide high binding capacity, low non-specific background, and work with many antibody species. These beads can be used manually with a magnetic stand or with automated platforms, such as the Thermo Scientific KingFisher Instruments. ChIP-validated and quality-guaranteed antibodies are also available for use with the Pierce Magnetic ChIP Kit.

Highlights:

  • Rapid – obtain purified DNA that is ready for quantitative PCR in about 8 hours

  • Efficient and reproducible – micrococcal nuclease digestion and nuclear lysis are highly optimized

  • Sensitive – obtain results with as few as 10,000 cells (1 x 104)

  • Low background – Pierce Protein A/G Magnetic Beads are blocked in a non-DNA-containing reagent to minimize background

  • Complete – optimized positive control reagents are included: RNA polymerase II antibody and GAPDH promoter PCR primers

Includes:

Kit contains reagents for chromatin preparation, IP, DNA purification, and positive controls (antibody and primers)

Requires:

In vivo crosslinker (such as formaldehyde), micro-tip sonicator (such as Misonix™ Sonicator 3000), ChIP qualified antibody of choice, PCR primers for DNA sequence of interest, PCR master mix (containing a dye such as SYBR™ Green, if qPCR is desired), and a qPCR instrument

Recommended for:

  • Determine sites of specific protein-DNA interactions on genomic DNA by PCR or ChIP-Seq
  • Monitor the effects of histone modifications or chemical agents on DNA binding

Specifications

Description Pierce ChIP-grade Protein A/G Magnetic Beads
Content And Storage Store in original container protected from direct sunlight in a dry, cool and well-ventilated area.
Product Line Pierce
Quantity 5 mL
Target Antibody
Type Magnetic Bead (ChIP grade)
Ligand Type Protein A/G
Sufficient For Approx. 250 Assays

Frequently Asked Questions (FAQs)

What are the differences between Protein A, Protein G, Protein A/G, and Protein L Ig-binding proteins?

Protein A, Protein G, and Protein A/G bind almost exclusively to the IgG class of antibodies, but their binding properties differ among species and subclasses of IgG. Protein L binds in the variable fragment of some kappa light chains and can react with any immunoglobulin, not just IgG, as long as the correct kappa light chains are present. Protein L does not bind lambda light chains and certain kappa chains of different species.

-Protein A is generally preferred for rabbit, pig, dog, and cat IgG.
-Protein G has better binding capacity for a broader range of mouse and human IgG subclasses (e.g., IgG1 vs. IgG2)
-Protein A/G is a recombinant fusion protein that includes the IgG-binding domains of both Protein A and Protein G. Therefore, Protein A/G is ideal for binding the broadest range of IgG subclasses from rabbit, mouse, human, and other mammalian samples.
-Protein L binds to certain immunoglobulin kappa light chains. Because kappa light chains occur in members of all classes of immunoglobulin (i.e., IgG, IgM, IgA, IgE and IgD), Protein L can purify these different classes of antibody. However, only those antibodies within each class that possess the appropriate kappa light chains will bind. Generally, empirical testing is required to determine if Protein L is effective for purifying a particular antibody. It binds only Vk1 in mouse and VkI, VkIII and VkIV in human.
Read more about the general characteristics of Ig-binding proteins (https://www.thermofisher.com/us/en/home/life-science/antibodies/antibody-purification-kits-reagents.html) and (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0034-Ab-binding-proteins.pdf).

For Research Use Only. Not for use in diagnostic procedures.