Thermo Scientific™ CHAPS Detergent (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate)

CHAPS is a sulfobetaine derivatives of cholic acid. This zwitterionic detergent is useful for membrane protein solubilization when it is important to maintain protein activity. CHAPS detergent is soluble over a wide range of pH (2 to 12) and are easily removed from solution by dialysis because of their high critical micelle concentration (CMC).

Highlights:

• Versatile zwitterionic detergents
• Provide mild but effective lysis of cultured mammalian cells
• Nondenaturing and generally do not inactivate protein functions
• Can be removed from solution by dialysis
• Commonly used for isoelectric focusing (IEF) and 2D electrophoresis

Properties of CHAPS detergent:

• Chemical Name: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
• Molecular Weight: 614.88
• Detergent Class: Zwitterionic
• Aggregation Number: 10
• Micelle Molecular Weight: 6149
• Critical Micelle Concentration (CMC): 8 to 10mM (0.4920 to 0.6150%, w/v)
• Cloud Point: ≥ 100°C
• Dialyzable: Yes