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  6. Thermo Scientific™ CHAPS Detergent (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate)

Thermo Scientific™ CHAPS Detergent (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate)

Catalog No. PI28299
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100 g
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PI28299 100 g
PI28300 5 g
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Catalog No. PI28299 Supplier Thermo Scientific™ Supplier No. 28299
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Description

Non-denaturing Zwitterionic detergents that disrupt nonspecific protein interactions with less protein aggregation than nonionic detergents.

Thermo Scientific™ CHAPS is a zwitterionic detergent that is especially well suited for protecting the native state of proteins.

CHAPS is a sulfobetaine derivatives of cholic acid. This zwitterionic detergent is useful for membrane protein solubilization when it is important to maintain protein activity. CHAPS detergent is soluble over a wide range of pH (2 to 12) and are easily removed from solution by dialysis because of their high critical micelle concentration (CMC).

Highlights:

  • Versatile zwitterionic detergents
  • Provide mild but effective lysis of cultured mammalian cells
  • Nondenaturing and generally do not inactivate protein functions
  • Can be removed from solution by dialysis
  • Commonly used for isoelectric focusing (IEF) and 2D electrophoresis

Properties of CHAPS detergent:

  • Chemical Name: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
  • Molecular Weight: 614.88
  • Detergent Class: Zwitterionic
  • Aggregation Number: 10
  • Micelle Molecular Weight: 6149
  • Critical Micelle Concentration (CMC): 8 to 10mM (0.4920 to 0.6150%, w/v)
  • Cloud Point: ≥ 100°C
  • Dialyzable: Yes

 

Specifications

Product Type Detergent
Content And Storage Store at Room Temperature
Form Powder
Reagent Type Detergent (Pure)
Quantity 100 g

Frequently Asked Questions (FAQs)

Are there mass spectrometry reagents for membrane proteins?

CHAPS (Cat. Nos. 28299, 28300), OTG (Cat. No. 28351) and Sodium Deoxycholate (Cat. Nos. 89904, 89905) are detergents often used to solubilize and extract membrane proteins from samples

For Research Use Only. Not for use in diagnostic procedures.