Thermo Scientific™ Pierce™ Centrifuge Columns

Description

Thermo Scientific™ Pierce Centrifuge Columns provide exceptional convenience, speed and performance for many types of small to medium scale affinity purification procedures.
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Pierce Centrifuge Columns are convenient tools for chromatography experiments involving 40μL to 10mL of beaded agarose or other affinity matrix. The columns are made from low protein binding polypropylene for compatibility with antibody and other protein purification, and they fit into standard centrifuge tubes for use in any centrifuge. Simply add the affinity resin to one of the-polypropylene columns, remove the twist-off bottom and allow the beads to settle to form the resin bed. Process sample by gravity-flow, then use a centrifuge to efficiently wash and elute purification products from the column.

Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use any combination of processing techniques to get the best possible chromatography results!

Highlights:

• Available in four sizes to accommodate 40μL to 10mL resin volumes
• Perform experiments using any combination of spin or gravity-flow processing
• Columns made of polypropylene with polyethylene filter discs, ensuring minimal protein-binding properties
• Columns fit into standard centrifuge tubes for sample collection and centrifugation in standard benchtop centrifuge rotors

0.8mL Centrifuge Columns (Part No. 89868, 89869)

• Total volume: 800μL
• Resin volume: 40 to 400μL
• Filter type: polyethylene filter, ∽30μm (20 to 50) pore size
• Receiver tube: fits standard microcentrifuge tubes
• Cap type: O-ring screw top caps; twist-off bottoms

2mL Centrifuge Columns (Part No. 89896)

• Total volume: 5mL (2mL resin bed, 3mL reservoir)
• Resin volume: 2mL
• Filter type: polyethylene filter, ∽30μm (20 to 50) pore size
• Receiver tube: fits standard 15mL conical centrifuge tubes
• Cap type: screw top caps; twist-off bottoms and press-on caps to reseal

5mL Centrifuge Columns (Part No. 89897)

• Total volume: 8mL (5mL resin bed, 3mL reservoir)
• Resin volume: 5mL Filter type: polyethylene filter, ∽30μm (20 to 50) pore size
• Receiver tube: fits standard 15mL conical centrifuge tubes
• Cap type: screw top caps; twist-off bottoms and press-on caps to reseal

10mL Centrifuge Columns (Part No. 89898)

• Total volume: 22mL (10mL resin bed, 12mL reservoir)
• Resin volume: 10mL
• Filter type: polyethylene filter, ∽30μm (20 to 50) pore size
• Receiver tube: fits standard 50mL conical centrifuge tubes
• Caps: screw top caps; twist-off bottoms and press-on caps to reseal

Column Extenders (Part No. 69707)

• Increase column reservoir volume by 35 to 40mL
• Designed especially for use in gravity-flow procedures
• Fit Part No. 89896, 89897, 89898 only