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Thermo Scientific™ Pierce™ CDI-activated Agarose Resin
Description
Thermo Scientific™ Pierce CDI-Activated Agarose is a porous, beaded chromatography support that is activated with carbonyldiimidazole groups for covalent immobilization of amine-containing affinity ligands.
Highlights:
Reliable coupling chemistry – immobilization occurs through the reaction of N-nucleophiles with 1,1'-carbonyldiimidazole groups of the resin to form stable, uncharged N-alkylcarbamate linkages
Easy to use – no secondary reagents needed; just wash equilibrate resin in alkaline coupling buffer and add ligand; reaction proceeds spontaneously
Stable activation – half-life of hydrolysis is much longer than hydroxysuccinimide ester activations, making immobilization reactions easier to prepare and control; simplifies filtration and washing before adding a ligand or protein
Well-defined coupling condition – reaction is most efficient with primary amines at pH 9 to 11
Properties of CDI-Activated Agarose:
- Resin: crosslinked 6% beaded agarose
- Activation: at least 50μmol 1,1'-carbonyldiimidazole (CDI) groups per milliliter of resin
- Format: supplied as stabilized, 50% slurry in acetone
Specifications
Specifications
| Content And Storage | Store at 4°C. |
| Product Line | Pierce |
| Quantity | 10 mL |
| Target | Primary Amine |
| Type | Resin |
| Stationary Phase | Carbonyl Diimidazole |
| Format | Bottle |
| Column Type | Affinity, Agarose Resin |
For Research Use Only. Not for use in diagnostic procedures.