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Thermo Scientific™ Pierce™ Boronic Acid Resin

Catalog No. pi20244
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Quantity:
10 mL
Catalog No. Quantity
PI20244 10 mL
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Catalog No. PI20244

Supplier: Thermo Scientific™ 20244

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Description

Selectively bind and remove or purify ribonucleotides and small oligonucleotide RNAs using polyacrylamide beads of immobilized m-aminophenyl-boronic acid.

Perform ribonucleotide and oligonucleotide RNA isolation with Thermo Scientific™ Pierce Boronic Acid Resin.

This immobilized boronic acid is an easy-to-use polyacrylamide affinity support for purification of ribonucleotides and other small molecular weight compounds that contain cis-diol groups. The ligand (m-aminophenyl-boronic acid) binds to the cis-diol groups on the sugar portion of nucleotides, forming a reversible five-member ring complex. After washing away non-bound molecules from the sample, the complex can be dissociated and the nucleotide eluted by lowering the pH or by addition of sorbitol.

Highlights:

  • Ligand – boronic acid

  • Support – beaded polyacrylamide (1800 MW exclusion limit)

  • Spacer – m-aminophenyl group

  • Loading – 100μmol boronate per milliliter of settled resin

  • Capacity – at least 99% binding and recovery of 110μmol AMP per milliliter of settled resin

  • Format – 50% resin slurry in 0.05% sodium azide

Specifications

Content And Storage Store at 4°C.
Form Resin Slurry
Product Line Pierce
Quantity 10 mL
Target Ribonucleotide
Type Resin
Stationary Phase Boronic Acid
Format Bottle
Column Type Affinity, Polyacrylamide Resin

For Research Use Only. Not for use in diagnostic procedures.