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Thermo Scientific™ Pierce™ Alkylating Reagents

Catalog No. PIA39270
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Quantity:
10 x 2 mg
30 x 9.3 mg
Includes:
Chloroacetamide
Iodoacetamide
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Catalog No. Quantity Includes
PIA39270 10 x 2 mg Chloroacetamide
PIA39271 30 x 9.3 mg Iodoacetamide
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Catalog No. PIA39270 Supplier Thermo Scientific™ Supplier No. A39270
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Description

Alkylating reagents provided in single-use, pre-weighed formats to streamline protein preparation workflows.

Thermo Scientific Pierce Iodoacetamide or Chloroacetamide are provided in a no-weigh format, where small amounts of reagent are pre-weighed and packaged for single use. This single-use format prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. The format enables use of a fresh vial of reagent each time, and eliminates the hassle of weighing out small amounts of reagent.

Alkylating reagents iodoacetamide and chloracetamide are sulfhydryl-reactive alkylating reagents used to block reduced cysteine residues for protein characterization and peptide mapping. Alkylation with iodoacetamide or chloracetamide after cysteine reduction results in the covalent addition of a carbamidomethyl group (57.07 Da) and prevents the formation of disulfide bonds. Reducing agents added after alkylation will react with excess iodoacetamide.

Specifications

Product Type Alkylating Reagent
Quantity 10 x 2 mg
Includes Chloroacetamide
Product Line Pierce

Frequently Asked Questions (FAQs)

How does a product with "No-Weigh" format reduce my concern over reagent stability?

The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html