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Thermo Scientific™ Pierce™ Agarose ChIP Kit

Numéro de catalogue. PI26156
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30 Reaction Kit
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Numéro de catalogue. Quantity
PI26156 30 Reaction Kit
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Numéro de catalogue. PI26156 Fournisseur Thermo Scientific™ Code fournisseur LSG26156
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Description

Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.

Thermo Scientific™ Pierce Agarose ChIP Kit provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

This chromatin IP kit contains sufficient reagents to perform 30 ChIP assays with appropriate controls using an optimized protocol and a convenient spin-column format. The kit includes a complete Chromatin Prep Module (also available separately) to efficiently extract and solubilize crosslinked DNA-protein interaction complexes from cells, plus an immunoprecipitation module that contains high-capacity, ChIP-grade Protein A/G agarose.

Highlights:

  • Simple and streamlined ChIP assay protocol (< 8 hours)
  • Highly efficient isolation and lysis of nuclei
  • Easy and reproducible enzymatic digestion
  • Low-background and high-capacity Protein A/G agarose resin
  • Highly specific positive control reagents included: RNA polymerase II antibody and GAPDH PCR primers
  • Fast and reproducible spin-column format; columns and collection tubes included
  • High-recovery DNA purification
  • Choose a complete ChIP assay kit or the separate chromatin preparation module

Includes:

Kit contains the Chromatin Prep Module (Part No. 26158; 11 components) for chromatin extraction and a complete set of immunoprecipitation reagents (15 components) for IP experiments with controls

Spécifications

Binding Capacity >50 mg Human IgG/mL resin binding capacity
Capacity (Metric) ≥50 mg Human IgG per mL Resin Binding
Description Pierce Agarose ChIP Kit
Content And Storage Sufficient For: 30 complete chromatin IP assay experiments• ChIP Grade Protein A/G Plus Agarose, 0.65 mL (store at 4°C)
• IP Dilution/Wash Buffer (5X), 11 mL (store at 4°C)
• IP Wash Buffer 3 (5X), 4.5 mL (store at 4°C)
• IP Elution Buffer (2X), 4.5 mL (store at 4°C)
• Column Accessory Pack, 3 packs (store at room temperature or 4°C)
• Microcentrifuge Tubes (1.5mL), 75 tubes (store at room temperature or 4°C)
• DNA Clean-Up Column, 40 columns (store at room temperature or 4°C)
• DNA Column Binding Solution, 30 mL (store at room temperature or 4°C)
• DNA Column Wash Solution, 6 mL (store at room temperature or 4°C)
• pH Indicator, 0.8 mL (store at room temperature or 4°C)
• DNA Column Elution Solution, 5 mL (store at room temperature or 4°C)
• Anti-RNA Polymerase II Antibody, 25 μL (store at -20°C)
• Normal Rabbit IgG (1 mg/mL), 10 μL (store at -20°C)
• ChIP Positive Control Primers (GAPDH promoter, Human-specific), 100 μL (store at -20°C)
• Pierce Chromatin Prep Module (Part No. 26158) (store Proteinase K and Micrococcal Nuclease at -20°C. Store all other components at 4°C).
Product Line Pierce
Quantity 30 Reaction Kit
Type ChIP Kit
Sufficient For 30 Reactions
Format Kit

For Research Use Only. Not for use in diagnostic procedures.