Thermo Scientific™ Phusion U Hot Start DNA Polymerases

Description

Thermo Scientific Phusion U DNA Polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion polymerase, Phusion U polymerase overcomes an important limitation of proofreading enzymes: it is able to incorporate dUTP and read through uracil present in DNA templates.

In addition to the uracil usage possibility, Phusion U polymerase features all the superior properties of other Phusion DNA polymerases: great accuracy, speed, ability to amplify long amplicons up to 20 kb, and a high specificity with Affibody-based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.

Phusion U Hot Start Green DNA Polymerase is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U polymerase performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.

Highlights

• Accuracy—high fidelity DNA polymerase (25X Taq)
• Uracil-tolerance—engineered to incorporate dUTP and amplify uracil containing templates
• Specificity—hot start for reduced nonspecific amplification and primer degradation
• Speed—short extension times (15–30 s/kb)
• Green format—permits direct loading of PCR products on gels

Applications

• Amplification of bisulphite-converted DNA
• Amplification of damaged or aged DNA
• Carry-over contamination control
• Uracil-excision based (USER) cloning methods

Using Phusion DNA polymerases

Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).