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Thermo Scientific™ Phusion™ HF and GC Buffer Packs

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

Supplier: Thermo Scientific™ F539L

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Catalog No. F539L

$53.50 / Each

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Quantity

Includes: Phusion GC Buffer Pack

5X Phusion GC Buffer (1X buffer provides 1.5 mM MgCl²)
50 mM MgCl2
DMSO

Phusion Green GC Buffer Pack

5X Phusion Green GC Buffer (1X buffer provides 1.5 mM MgCl₂)
50 mM MgCl₂
DMSO

Description

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10^-7) is lower than that in GC Buffer (9.5 x 10^-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

Specifications

Content And Storage	• 5X Phusion Green GC Reaction Buffer (4 x 1.5 mL) • 50 mM MgCl₂ (4 x 1.5 mL) • DMSO (0.5 mL) Store at –20°C.
Product Type	GC Buffer Pack
Shipping Condition	Wet or Dry Ice
Starting Material	DNA
Color	Green

Polymerase	Phusion High-Fidelity DNA Polymerase
Quantity	4 x 1.5 mL
For Use With (Application)	Standard PCR, High-fidelity PCR
Volume	4 x 1.5 mL

Product Suggestions Documents

Documents

Product Information

Manuals

User Guide: Phusion Green GC Buffer Pack Version: B.00 File size: 105.7 kB

Scientific Resources

Reference Materials

LabAid: Phusion High-Fidelity DNA Polymerases File size: 73.3 kB

For Research Use Only. Not for use in diagnostic procedures.