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Thermo Scientific™ Phusion Blood Direct PCR Master Mix

Amplify DNA directly from whole blood without prior purification

Supplier:  Thermo Scientific™ F175L

 View more versions of this product

Catalog No. F175L



Description

Description

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction. This extremely accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Master Mix an ideal choice even for demanding applications, such as cloning. Phusion Blood Direct PCR Master Mix also allows analysis of blood samples with different preservatives (e.g., heparin, EDTA, citrate), storage conditions and from various species.

Highlights
  • Direct PCR - sample is added directly to PCR reaction, therefore there is no need for time-consuming and expensive DNA purification steps
  • Dilution & Storage protocol available - allows tens of PCR reactions from one tiny sample and allows re-testing
  • Specially engineered Phire DNA polymerase - extremely short PCR protocol times
  • Master Mix format with premixed gel loading dye - minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel
Applications
  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing
Includes
  • 2X Phusion Blood Direct PCR Master Mix/li>
  • 50 mM EDTA (pH 8.0)
  • 50 mM MgCl2 solution
  • 100% DMSO
  • Universal control primer mix
  • Water, nuclease-free
  • O'GeneRuler Express DNA Ladder
Specifications

Specifications

Contains:
• 5 x 1 mL Phusion Blood Direct PCR Master Mix (2X)
• 40 μL Universal Control Primer Mix
• 0.5 mL 50 mM EDTA
• 1.5 mL 50 mM MgCl2
• 0.5 mL DMSO
• 4 x 1.25 mL Water, nuclease-free
• 50 μg O'GeneRuler Express DNA Ladder (sufficient for 100 applications)

Store at -20°C.
Direct PCR
Fast
Phusion
500 x 20 μL Reactions
500 x 20 L Reactions
SuperMix or Master Mix
DNA
High
Phusion Blood II DNA Polymerase
Yes
Direct PCR Master Mix
Built-In Hot Start
Blunt
Blood
SDS
Documents

Documents

Promotions

Promotions

For Research Use Only. Not for use in diagnostic procedures.