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Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit DFS Item

Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.

Supplier:  Gibco™ A14697

Catalog No. A14697


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Description

Description

  • Used as a Geneticin™ selectable expression plasmid for engineering stable cell lines
  • Full length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells
  • WPRE (woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression
  • TOPO™ cloning site for rapid and efficient (>85%) cloning of Taq-amplified PCR products
  • Neomycin resistance gene for selection of stable cell lines with Geneticin™
  • pUC origin for high copy replication and maintenance of the plasmid in E. coli
Specifications

Specifications

Ampicillin (AmpR)
Constitutive
Transfection
High Copy Plasmid
TA Cloning Kit
Untagged
pUC Origin
NeoR
pcDNA
TOPO™-TA
Store pcDNA™3.4-TOPO™ vector and all cloning reagents at -20°C.
Store One Shot™ TOP10 Chemically Competent E. coli cells at -80°C.
Protein Production, Constitutive Expression
TOPO™
CMV
20 μg Kit
Geneticin™ (G-418)
SV40 Promoter
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Product Information

Manuals

pcDNA3.4-TOPO TA Cloning Kit Product Insert Version: Rev 2.0 File size: 209.7 kB
pcDNA3.4-TOPO TA Cloning Kit User Guide Version: Rev 2.0 File size: 886 kB
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For Research Use Only. Not for use in diagnostic procedures.