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Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit

Catalog No. a14697
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20 μg Kit
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A14697 20 μg Kit
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Catalog No. A14697 Supplier Gibco™ Supplier No. A14697
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Description

Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.

  • Used as a Geneticin™ selectable expression plasmid for engineering stable cell lines
  • Full length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells
  • WPRE (woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression
  • TOPO™ cloning site for rapid and efficient (>85%) cloning of Taq-amplified PCR products
  • Neomycin resistance gene for selection of stable cell lines with Geneticin™
  • pUC origin for high copy replication and maintenance of the plasmid in E. coli

Specifications

Antibiotic Resistance Bacterial Ampicillin (AmpR)
Cloning Method TOPO™-TA
Constitutive or Inducible System Constitutive
Content And Storage Store pcDNA™3.4-TOPO™ vector and all cloning reagents at -20°C.
Store One Shot™ TOP10 Chemically Competent E. coli cells at -80°C.
Delivery Type Transfection
For Use With (Application) Protein Production, Constitutive Expression
Plasmid High Copy Plasmid
Product Line TOPO
Product Type TA Cloning Kit
Promoter CMV
Protein Tag Untagged
Quantity 20 μg Kit
Replication Origin pUC Origin
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Selection Marker Eukaryotic NeoR
Selection Marker Promoter SV40 Promoter
Vector pcDNA
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For Research Use Only. Not for use in diagnostic procedures.