OPTIZYME™ NotI, Fisher BioReagents™

Description

5'...G C^G G C C G C...3'
3'...C G C C G G^C G...5'

Conditions for 100% Activity

• 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl₂ , 100mM NaCl and 0.1mg/ml BSA.
• Incubate at 37°C.

Supplied with: 10X OPTIZYME Buffer 3
Enzyme Activity in OPTIZYME buffers

• Buffer 1: 0 - 20%
• Buffer 2: 20 - 50%
• Buffer 3: 100%
• Buffer 4: 0 - 20%
• Buffer 5: 20 - 50%

Storage Buffer:
20mM Tris-HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with NotI.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of NotI is required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Compatible Ends: Bsp120I, CfrI, Eco52I.
Methylation Effects:
Dam: never overlaps - no effect.
Dcm: never overlaps - no effect.
CpG: completely overlaps - blocked.

• GCGGCCGC

(Cleavage blocked)

EcoKI: never overlaps - no effect.

EcoBI: never overlaps - no effect.