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OPTIZYME™ NotI, Fisher BioReagents™

$387.33 - $410.67

Specifications

For Use With (Application) >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I
Concentration 10U/μL
Cut Site GC.GGCCGC
Format Liquid
Components 25 to 50% Water, >50% Glycerin
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
BP8004-5 View Documents Fisher BioReagents
BP80045
1000U Each for $387.33
 
BP8004-1 View Documents Fisher BioReagents
BP80041
300U Each for $410.67
 
Description

Description

5'...G C^G G C C G C...3'
3'...C G C C G G^C G...5'

Conditions for 100% Activity

  • 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 , 100mM NaCl and 0.1mg/ml BSA.
  • Incubate at 37°C.
Supplied with: 10X OPTIZYME Buffer 3
Enzyme Activity in OPTIZYME buffers
  • Buffer 1: 0 - 20%
  • Buffer 2: 20 - 50%
  • Buffer 3: 100%
  • Buffer 4: 0 - 20%
  • Buffer 5: 20 - 50%
Storage Buffer:
20mM Tris-HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with NotI.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of NotI is required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Compatible Ends: Bsp120I, CfrI, Eco52I.
Methylation Effects:
Dam: never overlaps - no effect.
Dcm: never overlaps - no effect.
CpG: completely overlaps - blocked.
  • GCGGCCGC
  • (Cleavage blocked)
  • EcoKI: never overlaps - no effect.
  • EcoBI: never overlaps - no effect.
  • Specifications

    Specifications

    >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I
    GC.GGCCGC
    25 to 50% Water, >50% Glycerin
    NotI
    Keep container tightly closed
    10U/μL
    Liquid
    7.4
    20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol