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Oncomine™ Myeloid Research Assay—Chef Ready

Catalog No. A36941
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32 reactions
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A36941 32 reactions
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Catalog No. A36941 Supplier Thermo Fisher Scientific Supplier No. A36941

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Specifications

Content And Storage Store in freezer (-5 to -30°C).
Sample Type DNA
Sequencing Type Genome and DNA Sequencing
For Use With (Equipment) Ion PGM™ System
Libraries Targeted Sequencing Library
No. of Reactions 32
Product Line Oncomine
Quantity 32 reactions
Shipping Condition Dry Ice
Workflow Step DNA Extraction

Frequently Asked Questions (FAQs)

What extraction kit is compatible with the Oncomine Myeloid Research Assay (Cat. Nos. A36940, A36941)?

We have validated the use of the MagMAX Total Nucleic Acid Isolation Kit (Cat. No. AM1840), which is compatible with whole blood and bone marrow samples. We recommend using your preferred commercially available kit to sequentially isolate high quality gDNA and RNA from research samples for use in library preparation.
What are the sample input requirements for the Oncomine Myeloid Research Assay - Chef-Ready (Cat. No. A36941?

10 ng of gDNA per pool at a minimum concentration of ≥0.833 ng/µL for Chef library preparation 10 ng of RNA at a minimum concentration ≥1.25 ng/µL for Chef library preparation
What sample types can I use with the Oncomine Myeloid Research Assay (Cat. Nos. A36940, A36941)?

Blood and bone marrow samples are compatible with the Oncomine Myeloid Research Assay. This kit is not intended for use with FFPE samples.
Which templating and sequencing kits are compatible with the Oncomine Myeloid Research Assay (Cat. Nos. A36940, A36941)?

The kit has been validated on the Ion GeneStudio S5 Series Systems and Ion PGM System.

With the Ion GeneStudio S5 Series Systems, we recommend using:
-Ion 510 & Ion 520 & Ion 530 Kit - Chef (Cat. No. A34019)
-Ion 530 Chip Kit (Cat. No. A27764).

With the Ion PGM System, we recommend using:
-Ion PGM Hi‑Q View OT2 Kit (Cat. No. A29900)
-Ion PGM Hi‑Q View Chef 400 Kit (Cat. No. A30798)
-Ion PGM Hi‑Q View Sequencing Kit (Cat. No. A30044)
-Ion 318 Chip v2 BC (Cat. No. 4488150)
Note: Ion 540 Chip is NOT compatible with the Oncomine Myeloid Research Assay as 400 base pair (850 Flows) sequencing is required.
How many samples can I multiplex with the Oncomine Myeloid Research Assay (Cat. Nos. A36940, A36941)?

The assay can be run on both the Ion PGM System and Ion S5/S5XL systems.
-PGM Plexy = 4 samples per Ion 318 Chip
-S5 Manual Library Prep Plexy = 12 samples per Ion 530 Chip
-S5 Chef Library Prep Plexy = 8 samples per Ion 530 Chip

What controls do you recommend using with the Oncomine Myeloid Research Assay (Cat. Nos. A36940, A36941)?

The recommended controls are:
-AcroMetrix Oncology Hotspot Control, Cat. No. 969056 (https://www.thermofisher.com/order/catalog/product/969056)
-Seraseq Myeloid Fusion RNA Mix, Cat. No. 0710-0407 (https://www.seracare.com/Seraseq-Myeloid-Fusion-RNA-Mix-0710-0407/)
-Seraseq Myeloid Mutation DNA Mix, Cat. No. 0710-0408 (https://www.seracare.com/Seraseq-Myeloid-Mutation-DNA-Mix-0710-0408/)
Is the Oncomine Myeloid Research Assay (Cat. No. A36940) compatible with the Ion Chef System for automated library preparation?

For automated library preparation using the Ion Chef System, we offer the Oncomine Myeloid Research Assay - Chef-Ready (Cat. No. A36941).
What is included in the Oncomine Myeloid Research Assay - Chef-Ready (Cat. No. A36941)?

The Oncomine Myeloid Research Assay - Chef-Ready (Cat. No. A36941) consists of the Oncomine Myeloid DNA Panel (2-pools) and the Oncomine Myeloid RNA Panel (1-pool) at 2X concentration pre-measured in barcoded Primer Pool tubes ready to load into an Ion AmpliSeq Chef Reagents DL8 cartridge. In addition, the kit provides all the reagents and supplies in an Ion AmpliSeq Kit for Chef DL8 (Cat. No. A29024) sufficient for preparing 32 libraries.
How many primer pools are in the Oncomine Myeloid Research Assay (Cat. Nos. A26940 and A36941)?

There are 3 primer pools, 2 DNA pools, and 1 RNA pool.
How many genes are covered by the Oncomine Myeloid Research Assay (Cat. Nos. A26940 and A36941)?

The panel is comprised of 40 key DNA genes and a broad fusion panel of 29 driver genes to cover the most relevant targets in major myeloid disorders such as acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML).
What is the Oncomine Myeloid Research Assay?

The Oncomine Myeloid Research Assay is a comprehensive, targeted NGS assay designed to assist in the understanding of myeloid cancer. Specifically, it interrogates all relevant DNA mutations and fusion transcripts associated with myeloid disorders in a single NGS run. The Oncomine Myeloid Research Assay, Cat. No. A36940, is for manual library preparation and Oncomine Myeloid Research Assay-Chef Ready, Cat. No. A36941, is for automated library preparation using the Ion Chef System.