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Invitrogen™ Novex™ TBE Running Buffer (5X)

Catalog No. LC6675
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LC6675 1 L
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Catalog No. LC6675 Supplier Invitrogen™ Supplier No. LC6675
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Description

Compatible with agarose and polyacrylamide gels

Novex™ TBE Gels provide highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands.

  • Prepared with 0.5X TBE as gel buffer
  • Sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions
  • TBE and DNA Retardation Gels are designed to run on the XCell SureLock™ Mini-Cell

Acrylamide Gel Electrophoresis, DNA & RNA Purification & Analysis, Nucleic Acid Gel Electrophoresis & Blotting

Order Info

Shipping Condition: Room Temperature

Specifications

Buffer Running Buffer
Concentration 5 X
Content And Storage Store at room temperature
Gel Compatibility Polyacrylamide Gels, Agarose Gels
For Use With (Application) Nucleic Acid Gel Electrophoresis, Blotting
Product Line Novex
Product Type TBE Running Buffer
Quantity 1 L
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

My Novex TBE buffer has precipitated out of solution. What can I do?

If a slight turbidity develops, the fine precipitate can be dissolved by autoclaving for 5 minutes at 110°C. Do not autoclave in the container supplied. This treatment has no deleterious effect on the buffering properties of TBE.

Do you have the recipe for the TBE running buffer and the Hi-Density TBE Sample Buffer?

Please see the recipes below:

TBE Running Buffer (5X):
Tris base, 54.0 g
Boric acid, 27.5 g
EDTA (free acid), 2.9 g
Deionized water to 1.0 L

Hi-Density TBE Sample Buffer (5X):
5X TBE Running buffer (Cat. No. LC6675), 2 mL
Ficoll Type 400, 1.5 g
Bromophenol blue, 1 mL of 1% solution
Xylene cyanol 1 mL of 1% solution
Deionized water to 10.0 mL

For Research Use Only. Not for use in diagnostic procedures.