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Invitrogen™ SUMO Protease

Catalog No. 12588018
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12588018 250 units
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Catalog No. 12588018 Supplier Invitrogen™ Supplier No. 12588018
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Description

Includes

SUMO Protease is provided with 10X high salt and 10X no salt dilution buffers.

A recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae

SUMO Protease, also known as Ulp, is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence.

  • Highly active cleavage
  • No non-specific proteolysis (highly specific cleavage)
  • Activity from 2°C to 37°
  • A six-histidine sequence to facilitate its removal from the digested protein sample

Application:Removal of fusion tags from recombinant proteins
Unit Definition: One unit of SUMO Protease is defined as the amount of enzyme needed to cleave 85% of 2μg of substrate protein at 30°C in one hour
Quality Control: SUMO Protease has greater than 85% single-band purity with no non-specific protease contamination; functionally tested for the absence of any non-specific protease activity

GST-Tagged Protein Purification, His-Tagged Protein Purification, Protein and Peptide Purification, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Dry Ice

Specifications

Host Species S. cerevisiae
Content And Storage SUMO Protease is provided with 10X high salt and 10X no salt dilution buffers. Store at -80°C. Guaranteed stable for 6 months when properly stored.
Shipping Condition Dry Ice
Quantity 250 units

Frequently Asked Questions (FAQs)

Are NP40 and DTT required for SUMO Protease activity? Will they interfere with downstream applications?

Yes, they are both required, although it might be possible to substitute the NP40 with another detergent via dialysis if you have concern about interference with mass spectrometry. DTT is an absolute requirement.
What enzyme should I choose if I do not want any extra amino acids left on my protein of interest after cleavage?

We would recommend using our SUMO system.
How does SUMO protease work?

SUMO protease, also known as Ulp, is a recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae. It is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence. The SUMO protease itself has a His tag for easy removal from the protein mix after cleavage.
What products do you offer for enzymatic cleavage of fusion tags from recombinant proteins?

We offer the following products:

-AcTEV Protease (Cat. Nos. 12575015, 12575023)
-EKMax Enterokinase (Cat. Nos. E18001, E18002)
-SUMO Protease (Cat. No. 12588018)

For Research Use Only. Not for use in diagnostic procedures.