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Invitrogen™ Novex™ Hi-Density TBE Sample Buffer (5X)

Catalog No. LC6678
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10 mL
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LC6678 10 mL
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Catalog No. LC6678 Supplier Invitrogen™ Supplier No. LC6678
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Description

Compatible with polyacrylamide gels

Novex TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions

  • Novex TBE and DNA Retardation Gels are designed to run on XCell SureLock™ Mini-Cell
  • Formulation: Novex TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS
  • Recommended Buffers: For optimum performance, Novex TBE Running Buffer and Novex Hi-Density TBE Sample Buffer are strongly recommended for use with these gels
  • Novex Hi-Density TBE Sample Buffer contains the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, and tracking dyes Bromophenol Blue and Xylene Cyanol

Acrylamide Gel Electrophoresis, DNA & RNA Purification & Analysis, Nucleic Acid Gel Electrophoresis & Blotting

Order Info

Shipping Condition: Wet Ice

Specifications

Buffer Sample Loading Buffer
Concentration 5 X
Content And Storage Store at 2°C to 8°C
Gel Compatibility Polyacrylamide Gels
For Use With (Application) Nucleic Acid Gel Electrophoresis, Blotting
Label or Dye Bromophenol Blue, Xylene Cyanol
Product Line Novex
Product Type TBE Buffer
Quantity 10 mL
Shipping Condition Wet Ice

Frequently Asked Questions (FAQs)

My Novex TBE buffer has precipitated out of solution. What can I do?

If a slight turbidity develops, the fine precipitate can be dissolved by autoclaving for 5 minutes at 110°C. Do not autoclave in the container supplied. This treatment has no deleterious effect on the buffering properties of TBE.

Do you have the recipe for the TBE running buffer and the Hi-Density TBE Sample Buffer?

Please see the recipes below:

TBE Running Buffer (5X):
Tris base, 54.0 g
Boric acid, 27.5 g
EDTA (free acid), 2.9 g
Deionized water to 1.0 L

Hi-Density TBE Sample Buffer (5X):
5X TBE Running buffer (Cat. No. LC6675), 2 mL
Ficoll Type 400, 1.5 g
Bromophenol blue, 1 mL of 1% solution
Xylene cyanol 1 mL of 1% solution
Deionized water to 10.0 mL

For Research Use Only. Not for use in diagnostic procedures.