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Invitrogen™ Novex™ Aβ42 Human ELISA Kit DFS Item

Quantifies natural and recombinant Aβ42 selectively in tissue culture medium, tissue homogenate, cerebrospinal fluid, and other samples

$720.30 - $1,461.60

Specifications

Concentration 15.6-1000pg/mL
Conjugate HRP (Horseradish Peroxidase)
Gene ID (Entrez) 351
Incubation Time 4 hr.
Marker Gene Aβ 42
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Includes:  Pre-coated plate, standard, detector antibody, anti-IgG-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

KHB3441

DFS Item
 
invitrogen™
KHB3441
96 assays Each for $720.30

KHB3442

DFS Item
 
invitrogen™
KHB3442
192 assays Each for $1,461.60
Description & Specifications

Specifications

Concentration 15.6-1000pg/mL
Conjugate HRP (Horseradish Peroxidase)
Gene ID (Entrez) 351
Incubation Time 4 hr.
Marker Gene Aβ 42
Sample Type Cerebrospinal Fluid, Serum, Tissue Homogenates
Sample Volume 50μL
Sensitivity <10pg/mL
Species Human
Target amyloid beta (A4) precursor protein

Easy-to-run sandwich ELISA
The Human Aβ42 kit is a solid-phase sandwich enzyme-linked immuno-sorbent assay (ELISA). A monoclonal antibody specific for the NH2-terminus region of human Aβ has been coated onto the wells of the microtiter strips provided. During the first incubation, standards, controls, and unknown samples are pipetted into the wells and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1-42 Aβ sequence. Bound rabbit antibody is detected through the use of a horseradish peroxidase-labeled anti-rabbit antibody. After washing, horseradish peroxidase-labeled anti-rabbit antibody (enzyme) is added. After a second incubation and washing to remove all unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of human Aβ42 present in the original specimen. Samples are quantitated using a standard curve generated from the recombinant Aβ42 included with the kit.

Results typically in one day—rapid four-hour incubation protocol
The kit comes with the capture antibody pre-coated to the wells, eliminating the need to coat the plate. No overnight incubation needed. The incubation steps can be performed in four hours.

Large dynamic range: 7.1–1,000 pg/mL
The large dynamic range of the assay minimizes the need to guess the dilution needed for your sample to fall within the linear assay range.

Economical, removable wells
The precoated wells come as removable 8-well strips, allowing assay size to tailored to needs. Two standard vials per plate provided allowing the option of reconstituting a new vial should if the entire plate is not run at once.

  • Easy-to-run sandwich ELISA
  • Results typically in one day—rapid four-hour incubation protocol
  • Large dynamic range—7.1–1000pg/mL
  • Pre-coated, removable 8-well strips
  • Rigorous validation
  • Each ELISA kit is validated for sensitivity, specificity, and lot-to-lot consistency

This kit does not detect Aβ40 or Aβ43. See protocol insert for more information on validation.

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay

Shipping condition: Wet ice

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