Easy-to-run sandwich ELISA
The Human Aβ42 kit is a solid-phase sandwich enzyme-linked immuno-sorbent assay (ELISA). A monoclonal antibody specific for the NH2-terminus region of human Aβ has been coated onto the wells of the microtiter strips provided. During the first incubation, standards, controls, and unknown samples are pipetted into the wells and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1-42 Aβ sequence. Bound rabbit antibody is detected through the use of a horseradish peroxidase-labeled anti-rabbit antibody. After washing, horseradish peroxidase-labeled anti-rabbit antibody (enzyme) is added. After a second incubation and washing to remove all unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of human Aβ42 present in the original specimen. Samples are quantitated using a standard curve generated from the recombinant Aβ42 included with the kit.
Results typically in one day—rapid four-hour incubation protocol
The kit comes with the capture antibody pre-coated to the wells, eliminating the need to coat the plate. No overnight incubation needed. The incubation steps can be performed in four hours.
Large dynamic range: 7.1–1,000 pg/mL
The large dynamic range of the assay minimizes the need to guess the dilution needed for your sample to fall within the linear assay range.
Economical, removable wells
The precoated wells come as removable 8-well strips, allowing assay size to tailored to needs. Two standard vials per plate provided allowing the option of reconstituting a new vial should if the entire plate is not run at once.
- Easy-to-run sandwich ELISA
- Results typically in one day—rapid four-hour incubation protocol
- Large dynamic range—7.1–1000pg/mL
- Pre-coated, removable 8-well strips
- Rigorous validation
- Each ELISA kit is validated for sensitivity, specificity, and lot-to-lot consistency
This kit does not detect Aβ40 or Aβ43. See protocol insert for more information on validation.
Cell Analysis, Immunoassays, Ready-To-Use Immunoassay
Shipping condition: Wet ice
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