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MP Biomedicals Proteinase K from Tritirachium album


Highly active stable endopeptidase with a broad spectrum of action

Manufacturer: mp biomedicals  0219398180

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Catalog No. ICN19398180

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Description & Specifications

Specifications

Product Name Proteinase K
Format Lyophilized Powder
Color White
Grade Molecular biology reagent
Inhibitors Diisopropylfluorophosphate or Phenylmethane Sulfonyl Fluoride, AEBSF and Trypsin
Source Tritirachium album
pH 7.5 to 12
Molecular Weight 30000
Quantity 100mg
For Use With (Application) Useful for the proteolytic inactivation of Nucleases during the isolation of DNA and RNA
Storage Requirements 4°C

Proteinase K is used in digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA, as well as in removal of endotoxins bound to cationic proteins such as lysozyme and ribonuclease A and in determination of enzyme localization on membranes.

  • Grade: Molecular biology reagent
  • Specific Activity: >30 mAnson u/mg
  • Presentation: White powder
  • Isoelectric Point: 8.9 (Lit.)
  • Extinction Coefficient: E1%= 14.2 (280 nm,10 mM NaCl and 5 mM CaCl2, pH 8.0) (Lit.)
  • pH: 7.5 -12.00 (denatured hemoglobin as substrate) (Lit.)
  • Soluble in water

One Anson unit is the amount of enzyme which will liberate 1 °mole of folin-positive amino acids per minnute at pH 7.5 and 35°C using hemoglobin as substrat

Isolation of DNA and RNA