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  6. Invitrogen™ Mouse anti-Rat IgG2a Secondary Antibody, eFluor™ 660, eBioscience™

Invitrogen™ Mouse anti-Rat IgG2a Secondary Antibody, eFluor™ 660, eBioscience™

Catalog No. 501124373
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100 μg
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501124373 100 μg
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Catalog No. 501124373 Supplier Invitrogen™ Supplier No. 50481782
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Description

Mouse Monoclonal Secondary Antibody

Description: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies. Applications Reported: This r2a-21B2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This r2a-21B2 antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells stained with a rat IgG2a primary antibody. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Rat IgG2a
Applications Flow Cytometry
Classification Monoclonal
Clone r2a-21B2
Concentration 0.2 mg/mL
Conjugate eFluor 660
Formulation PBS with 0.09% sodium azide; pH 7.2
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Rat
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Isotype IgG1 κ
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