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  6. Invitrogen™ Mouse anti-Rat IgG2a Secondary Antibody, eFluor™ 615, eBioscience™

Invitrogen™ Mouse anti-Rat IgG2a Secondary Antibody, eFluor™ 615, eBioscience™

Catalog No. 501130717
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25 μg
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501130717 25 μg
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Catalog No. 501130717 Supplier Invitrogen™ Supplier No. 42481780
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Description

Mouse Monoclonal Secondary Antibody

Description: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies. Applications Reported: This R2a-21B2 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This R2a-21B2 antibody has been tested by immunocytochemistry and immunohistochemistry using a rat primary antibody at 10 μg/mL. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor™ 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 μm post-manufacturing filtered.

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Rat IgG2a
Applications Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry
Classification Monoclonal
Clone r2a-21B2
Concentration 0.2 mg/mL
Conjugate eFluor 615
Formulation PBS with 0.09% sodium azide; pH 7.2
Host Species Mouse
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Rat
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Isotype IgG1 κ
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