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  6. Invitrogen™ Mouse anti-Rat IgG2a Secondary Antibody, eFluor™ 570, eBioscience™

Invitrogen™ Mouse anti-Rat IgG2a Secondary Antibody, eFluor™ 570, eBioscience™

Catalog No. 501123626
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25 μg
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501123626 25 μg
501123627 100 μg
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Catalog No. 501123626 Supplier Invitrogen™ Supplier No. 41481780

Description

Mouse Monoclonal Secondary Antibody

Description: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies. Applications Reported: This R2a-21B2 antibody has been reported for use in immunocytochemistry, and immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections. Applications Tested: This R2a-21B2 antibody has been tested by immunocytochemistry on fixed and permeabilized cells that have been stained first with a rat IgG2a anti-mouse antibody. This can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nm.

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Rat IgG2a
Applications Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry
Classification Monoclonal
Clone r2a-21B2
Concentration 0.2 mg/mL
Conjugate eFluor 570
Formulation PBS with 0.09% sodium azide; pH 7.2
Host Species Mouse
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Rat
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Isotype IgG1 κ
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