Invitrogen™ Mouse anti-Rat IgG1 Secondary Antibody, FITC, eBioscience™

Mouse Monoclonal Secondary Antibody

Description: The R1-12D10 monoclonal antibody recognizes rat IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG1. Applications Reported: This R1-12D10 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry. Applications Tested: This R1-12D10 antibody has been tested by flow cytometric analysis of cells stained with a rat IgG1 primary antibody and can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. This R1-12D10 antibody has also been tested by immunohistochemistry of human tissue stained with a rat IgG1 primary antibody and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.