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Invitrogen™ Mouse anti-Rat IgG1 Secondary Antibody, eFluor™ 615, eBioscience™

Catalog No. 501129216
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100 μg
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501129216 100 μg
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Catalog No. 501129216 Supplier Invitrogen™ Supplier No. 42481282

Description

Mouse Monoclonal Secondary Antibody

Description: The R1-12D10 monoclonal antibody recognizes rat IgG1 antibodies and can be used as a second step reagent in microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG1. Applications Reported: This R1-12D10 antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This R1-12D10 antibody has been tested by immunohistochemistry on frozen mouse tissue and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 μm post-manufacturing filtered.

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Rat IgG1
Applications Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry
Classification Monoclonal
Clone R1-12D10
Concentration 0.2 mg/mL
Conjugate eFluor 615
Formulation PBS with 0.09% sodium azide; pH 7.2
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Rat
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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