Invitrogen™ Mouse anti-Human IgG4 Fc Secondary Antibody, HRP

Description

MA1-82201 detects the Fc portion of human IgG4. No cross-reactivity is observed with human IgG1, IgG2, and IgG3 isotypes. A suggested histology positive control is tonsil tissue. This antibody reacts with Human, African green monkey, Cynomolgus monkey, Baboon, Chimpanzee and Rhesus Monkey IgG. Mouse anti Human IgG4 antibody, clone HP6025 recognizes the heavy chain of human IgG4, at an epitope in the Fc region. No cross-reactivity is observed with IgG1, IgG2, IgG3, IgM, IgA (Jefferis et al. 1985).

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.