missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Secondary Antibodies
  5. Mouse Secondary Antibodies
  6. PI31420

Invitrogen™ Mouse anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP

Catalog No. PI31420
Change view
Click to view available options
Quantity:
1.5 mL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PI31420 1.5 mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PI31420 Supplier Invitrogen™ Supplier No. 31420

Description

Mouse Polyclonal Secondary Antibody

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31420 has been successfully used in Western blot, and ICC applications. Antibody Specificity: Based on immunoelectrophoresis, the antibody reacts with the heavy chains on human IgG and with light chains common to most human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody exhibits minimal inherent cross-reactivity to mouse serum proteins. It has been passed through solid-phase immunoadsorbent gel to minimize any actual cross-reaction with mouse serum proteins. However, these antibodies may cross-react with immunoglobulins from other species. Restoration and Storage: Store product at 4°C until opened. Restore with 1.5 mL distilled water (0.8 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as undiluted liquid. After dilution, do not use for more than one day. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Country of Origin: USA.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Human IgG (H+L) Cross-Adsorbed
Applications Immunohistochemistry, Western Blot, Immunocytochemistry
Classification Polyclonal
Concentration 0.8 mg/mL
Conjugate HRP
Formulation PBS with 15mg/mL BSA and no preservative; pH 7.6
Host Species Mouse
Purification Method Antigen affinity chromatography
Quantity 1.5 mL
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Human
Content And Storage 4°C
Product Type Secondary Antibody
Form Lyophilized
Isotype IgG
Show More Show Less