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Applied Biosystems™ MicroSEQ™ ID Purification Combo Kit v2.0, with clean-up plates

Catalog No. A35852
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Catalog No. A35852 Supplier Applied Biosystems™ Supplier No. A35852
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Description

The MicroSEQ ID Purification Combo Kit v2.0 includes reagents for post-PCR and post-sequencing clean-up in the MicroSEQ ID microbial identification workflow.

The MicroSEQ ID Purification Combo Kit v2.0 includes reagents for post-PCR and post-sequencing clean-up in the MicroSEQ ID microbial identification workflow. This version contains one tube of ExoSAP-IT Express Reagent for post-PCR clean-up and two 96- well MicroSEQ ID Ultra Sequencing Clean-up Plates for post-sequencing clean-up.

ExoSAP-IT Express Reagent removes unincorporated dNTPs and primers from the post-amplification PCR mix in 5 minutes compared to 30 minutes with the original ExoSAP-IT Reagent while producing equivalent results. The MicroSEQ ID Ultra Sequencing Clean-up kit in 96-well plate or 8-strips format provides a convenient and simple way to remove dye terminators, dNTPs, salts, and residual primers from cycle sequencing reactions. The end products can be used directly for sequencing on any Applied Biosystems genetic analyzer.

Comparability studies demonstrated equivalent results between the original ExoSAP-IT Reagent and the improved ExoSAP-IT Express Reagent coupled with the MicroSEQ ID Ultra Sequencing Clean-Up kit in the MicroSEQ ID workflow (see figure below). Both ExoSAP-IT and ExoSAP-IT Express enzyme-treated PCR samples produced very similar sequencing results and the correct identification result.

ExoSAP-IT Express Reagent can be used downstream of the following PCR amplification kits:

  • MicroSEQ 500 16S rDNA PCR Kit
  • MicroSEQ Full Gene 16S rDNA PCR Kit
  • MicroSEQD2 rDNA Fungal PCR Kit

MicroSEQ ID Ultra Sequencing Clean-up Plates can be used downstream of the following sequencing kits:

  • MicroSEQ 500 16S rDNA Sequencing Kit
  • MicroSEQ Full Gene 16S rDNA Sequencing Kit
  • MicroSEQ D2 rDNA Fungal Sequencing Kit

Specifications

Content And Storage • 2 x ExoSAP-IT Express Reagent (100 reactions). Store at -5°C to -30°C.
• 2 x MicroSEQ ID Ultra Sequencing Clean-up Plate (96-well). Store at 2°C to 8°C.
Format Plate
Sample Type Bacteria, DNA, dsDNA, Fungi
For Use With (Application) Sequencing, PCR
High-throughput Compatibility High-throughput Compatible, Manual Protocols
No. of Reactions 100 Reactions
Product Line MicroSEQ
Quantity 1 kit
Shipping Condition Approved for shipment on Wet or Dry Ice
Target Fluorescent Dye Removal, DNA from Enzymatic Reactions (e.g. PCR)

Frequently Asked Questions (FAQs)

What were the results of comparability studies between the original ExoSAP-IT Reagent and the improved ExoSAP-IT Express Reagent provided with the MicroSEQ ID Purification Combo Kit v2.0?

Samples treated with the ExoSAP-IT and ExoSAP-IT Express reagents produced very similar sequencing results and the correct identification results.

Is ExoSAP-IT Express Reagent available as a stand-alone product?

Yes. The unit sizes available range from 480 to 2,000 reactions (https://www.thermofisher.com/order/catalog/product/75001.1.EA).
What is the minimum reaction volume to be used with the MicroSEQ ID Ultra Sequencing Clean-up 8-Strips Kit, Cat. No. A33246?

The minimum reaction volume is 5 µL. The reaction volume should be brought to at least 5 µL with distilled water before adding to the 8-well strips.
How long are the MicroSEQ ID Ultra Sequencing Clean-up 8-Strips good for once opened?

Strips should be used within 2 weeks of opening. We recommend storing unused strips from the 96-well plate holder, in the foil package, sealing and storing at 4 degrees C.
What are the storage conditions for the MicroSeq ID Purification Combo Kit v2.0, with clean-up plates?

Here are the storage conditions:
-ExoSAP-IT Express Reagent: Store at -5 to -30 degrees C
-MicroSEQ ID Ultra Sequencing Clean-up Plate: Store at 2-8 degrees C in a non-frost free freezer
How is the MicroSEQ ID Purification Combo Kit v2.0 shipped?

It is shipped on wet or dry ice.
What is the catalog number for the MicroSeq ID Purification Combo Kit v2.0, with clean-up plates?

It is Cat. No. A35852.
What is included with the MicroSEQ ID Purification Combo Kit v2.0, with clean-up plates?

Here are the components:
-ExoSAP-IT Express Reagent (one tube, sufficient for 100 reactions)
-MicroSEQ ID Ultra Sequencing Clean-up Plate (one 96-well plate, sufficient for 96 reactions)
Will primer-dimers affect the efficiency of ExoSAP-IT Express Reagent used in the MicroSEQ ID Purification Combo Kit v2.0?

Yes, ExoSAP-IT Express Reagent cannot digest primer-dimers because they are double-stranded.
What is the difference between ExoSAP-IT Reagent vs ExoSAP-IT Express Reagent included in the MicroSEQ ID Purification Combo Kit v2.0?

The difference is in the incubation time. ExoSAP-IT Reagent has a 15 min incubation step at 37 degrees C and another 15 min incubation at 80 degrees C. ExoSAP-IT Express Reagent has a total 5 min protocol with a 4 min incubation step at 37 degrees C followed by a 1 min incubation at 80 degrees C.

For Research Use Only. Not for use in diagnostic procedures.