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Thermo Scientific™ Micrococcal Nuclease (300 U/μL)
Digest single-stranded and double-stranded DNA and RNA with Thermo Scientific™ Micrococcal Nuclease (S7 Nuclease), an endo-exonuclease.
Supplier: Thermo Scientific™ EN0181
Description
Micrococcal Nuclease (S7 Nuclease) is a relatively nonspecific endo-exonuclease that digests single-stranded and double-stranded nucleic acids, but is more active on single-stranded substrates. Cleavage of DNA or RNA occurs preferentially at AT or AU-rich regions, yielding mononucleotides and oligonucleotides with terminal 3'-phosphates. The enzyme's activity is strictly dependent on Ca2+.
Applications
- Hydrolysis of nucleic acids in crude cell-free extracts
- Sequencing of RNA
- Studies of chromatin structure
- A model for protein folding and for structure-function studies
Specifications
| 300 U/μL | |
| Nuclease | |
| 8,000 units | |
| Micrococcal Nuclease |
For Research Use Only. Not for use in diagnostic procedures.