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Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix
Provide one-tube master mix for convenient and consistent cDNA synthesis for two-step RT-qPCR applications. The format helps streamline the RT-qPCR workflow, saves time, reduces pipeting errors to give customers sensitive, reliable and fast cDNA synthesis.
Manufacturer: Thermo Scientific™ M1661
Description
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT), an engineered RNase H minus enzyme, is formulated into convenient one-tube master mix with a simplified protocol, enabling consistency and maximum control of RT-qPCR. It was developed through molecular evolution to improve thermostability, robustness and synthesis rates. Maxima H Minus RT also shows up to a 50x increase in processivity, has no RNase H activity, and demonstrates higher sensitivity.
- Efficiently transcribes large and small amounts of RNA
- Quick and convenient setup eliminates pipeting caused variability
- Reduces risk of cross-contamination between samples
- Consistently higher sensitivity for wide variety of gene targets
- High linearity in cDNA synthesis over a broad dynamic range
- Complete enzyme activity from 42° to 55°C
Specifications
RT-PCR | |
first-strand cDNA | |
50 Reactions | |
Master Mix | |
RNA | |
RNA | |
200 μL | |
H Minus cDNA Synthesis Master Mix Kit | |
Maxima™ | |
Maxima H Minus | |
500 Reactions |
5X | |
Tube | |
RT-PCR | |
Reverse Transcription | |
up to 20 kb | |
Reverse Transcription | |
Real-Time PCR | |
50°C inculbation temperature for RT reactions | |
cDNA Synthesis Master Mix | |
Dry Ice |