Learn More
Applied Biosystems™ MagMAX™ Total Nucleic Acid Isolation Kit
Description
The MagMAX™ Total Nucleic Acid Isolation Kit includes zirconia beads for the mechanical disruption of difficult pathogens. RNA purified using the kit is ideal for PCR and RT-PCR applications. The kit uses magnetic bead–based purification and includes sufficient reagents for 100 reactions.
- Complete, ready-to-use reagents for RNA and DNA purification from tough-to-lyse bacteria
- Highly effective sample homogenization using bead beating and chemical lysis
- Magnetic bead–based purification technology allows effective removal of PCR inhibitors
- Nucleic acid purification is easily scalable and suitable for automation
Proven with a Wide Range of Samples:
- Biological Samples
- Feces and whole blood
- Biofluids such as milk, urine, nasal fluids, swab samples, semen, sputum, and spent culture medium
- Bacterial cultures and mammalian cell cultures
- Environmental Samples
- River and pond water
- Agricultural samples such as manure pits and cow alleyways
Benefits of the MagMAX™ Total Nucleic Acid Isolation Kit:
The MagMAX™ Total Nucleic Acid Isolation Kit procedure combines an effective method sample disruption with magnetic bead–based nucleic acid isolation and purification technology. The MagMAX™ microspherical paramagnetic beads have a large available binding surface and can be fully dispersed in solution, allowing thorough nucleic acid binding, washing, and elution. This results in consistent yields of high-quality nucleic acid with >50% recovery and little sample-to-sample variation (recovery may vary depending on sample type). The entire procedure can be completed in approximately 1 hour.
Overview of the Procedure:
Samples are disrupted using zirconia beads in a guanidinium thiocyanate–based solution; this rapidly releases nucleic acid while simultaneously inactivating nucleases. Samples are then diluted with isopropanol, and paramagnetic beads are added to the sample. The beads bind nucleic acids and are then immobilized on magnets and washed to remove proteins and other contaminants. A second wash solution is used to remove residual binding solution. Finally, nucleic acid is eluted in a small volume of low-salt buffer. The MagMAX™ Total Nucleic Acid Isolation Kit procedure is designed for processing samples in 96-well plates, thus 96 samples can be processed at once; however, the kit can also be used with fewer than 96 samples.
Accessory Products:
Lysis/Binding Solution Concentrate (Cat. No. AM8500), Wash Solution 1 Concentrate (Cat. No. AM8504), and Wash Solution 2 Concentrate (Cat. No. AM8640) are components of the MagMAX™ Total Nucleic Acid Isolation Kit that may also be purchased separately.
Specifications
Specifications
| Content And Storage | • Carrier NA and Lysis/Binding Enhancer, store at -20°C |
| Format | 96-well Plate |
| Isolation Technology | Magnetic Bead |
| Sample Type | Bacteria, Blood, Cells, Liquid Samples (e.g. Serum) |
| Final Product Type | RNA, DNA |
| For Use With (Application) | Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Southern Blotting, PCR, Northern Blotting |
| High-throughput Compatibility | High-throughput Compatible |
| Product Line | MagMAX |
| Quantity | 100 preps |
| Target | Total RNA, Total Nucleic Acids, Genomic DNA |
| Show More |
Frequently Asked Questions (FAQs)
Only MagMax96 for Microarrays Total RNA Isolation Kit (Cat. No. AM1839) is compatible, since it uses Trizol reagent and most of the salt carry-over from RNAlater reagent is eliminated during the lysis steps and partitioned from the aqueous RNA layer on top. For other MagMax kits, optimization is needed. High salt carry over is not optimal for MagMax RNA isolation steps.
The following components from the MagMAX Total Nucleic Acid Isolaton Kit can be purchased as standalone products and here are the details:
- NA Binding Beads: Can be purchased separately as DNA Binding Beads for MagMAX-96 DNA Multi-Sample Kit, Cat. No. 4489112 (note that these beads are the same as the beads in Cat. No. AM1840, but the fill volume differs)
- Lysis/binding Solution Concentrate: Can be purchased separately as MagMAX Lysis/Binding Solution Concentrate, Cat. No. AM8500
- Wash Solution 1 Concentrate: Can be purchased separately as MagMAX Wash Solution 1 Concentrate, Cat. No. AM8504
- Wash Solution 2 Concentrate: Can be purchased separately as MagMAX Wash Solution 2 Concentrate, Cat. No. AM8640
No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.
For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.
Yes, this can be done by sample splitting. Set the protocol so that the magnet will collect samples from multiple wells before continuing to the next step. Be sure not to overload.
For Veterinary Use Only.