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  5. Applied Biosystems™ MagMAX™ HMW DNA Kit and Booster

Applied Biosystems™ MagMAX™ HMW DNA Kit and Booster

Catalog No. A36624
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Format:
Kit
Reagent
No. of Reactions:
100 Preps
1000 Preps
Up to 100 Preps
Sample Type:
Fresh or Frozen Blood
Fresh or Frozen Blood, Tissue, Cells
Tissue, Cells
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Catalog No. Format No. of Reactions Sample Type
A36624 Reagent 100 Preps Fresh or Frozen Blood, Tissue, Cells
A36625 Reagent 1000 Preps Fresh or Frozen Blood, Tissue, Cells
A36626 Kit Up to 100 Preps Fresh or Frozen Blood
A36627 Kit Up to 100 Preps Tissue, Cells
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Catalog No. A36624 Supplier Applied Biosystems™ Supplier No. A36624
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Description

Maximize the full potential of your long-read sequencing applications with our MagMAX HMW DNA Kit. Designed for reproducibility and efficiency, it provides high-integrity DNA with large intact fragments from diverse samples. Suitable for both manual and automated protocols, it meets the varied needs of your laboratory with minimal processing time.

The MagMAX HMW DNA Kit is a magnetic bead-based nucleic acid purification kit for isolation of high-quality, high molecular weight (HMW) DNA from whole blood, cells, and tissue. This versatile kit is optimized for both automated workflows with KingFisher purification systems and manual isolation processes, providing flexibility and scalability. It is specifically designed to obtain intact DNA fragments larger than 100 kb, making it excellent for long-read sequencing and various downstream molecular applications.

The MagMAX HMW DNA Kit is for use with fresh and frozen whole blood, while the MagMAX HMW DNA Kit with Cell and Tissue Extraction Buffer is also compatible with cell and tissue sample types. Already have MagMAX DNA Multi-Sample Ultra 2.0 and/or MagMAX Cell and Tissue Extraction Buffer? No problem, we offer MagMAX HMW DNA Booster separately in the 100 prep size found in the kit or a 1000 prep size for higher throughput.

Features of the MagMAX HMW DNA Kit include:

  • Reproducibility and efficiency—consistently yields intact DNA fragments exceeding 100 kb from a range of sample types
  • Versatile solution—flexible workflows for low- to high-throughput sample processing with manual or automated protocols
  • Fast and optimized workflow—eliminates the need for post-processing steps or overnight elution
  • Innovate your research—optimal performance with long-read systems such as Oxford Nanopore Technologies and PacBio systems
  • Automation compatibility
  • Available as stand-alone products—reagents available in larger volume bottles sold separately to meet your scalability needs

Versatility for downstream applications

Start your workflow out right with the MagMAX HMW DNA Kit, designed to meet the high demand for high-quality nucleic acid isolation, a vital component for highly sensitive downstream applications. The MagMAX HMW DNA Kit has optimized workflows available with the KingFisher purification systems, enabling a semi-automated workflow with a total turnaround time of less than two hours. This versatile kit is also optimized for lower throughput manual workflows, making it an excellent choice for laboratories of all sizes and throughput needs. Experience the perfect blend of efficiency, flexibility, and reliability with the MagMAX HMW DNA Kit for your downstream molecular applications, such as long-read sequencing.

Isolate HMW DNA from multiple sample types with a single sample prep kit

The MagMAX HMW DNA Kit leverages the innovative MagMAX HMW DNA Booster to enhance the elongation of fragment size. It is designed to isolate large fragment gDNA (100–300 kb) from various sample types, including 200 μL of fresh or frozen whole blood, one million cultured cells, and 2–8 mg tissue samples. Dynabeads magnetic beads with optimized surface chemistry and high specific surface area enable efficient kinetics and high sensitivity in capturing nucleic acids. The robust chemistry and workflow design minimizes impurities, such as proteins and detergents, that can interfere with downstream molecular analysis. Finally, the HMW genomic DNA is released from the beads using a low quantity TE elution solution.

The MagMAX HMW DNA Kit leverages the MagMAX DNA Multi-sample Ultra 2.0 Kit chemistry. This kit is specifically designed for the extraction of high molecular weight DNA from fresh and frozen whole blood samples. For cell and tissue samples, the MagMAX HMW DNA Kit with Cell and Tissue DNA Extraction Buffer provides an optimized solution, delivering reliable and efficient nucleic acid isolation. Existing users of the MagMAX DNA Multi-sample Ultra 2.0 Kit (A36570) can enhance their workflow by incorporating the MagMAX HMW DNA Booster (A36624, A36625) for whole blood, along with the MagMAX Cell and Tissue DNA Extraction Buffer (A45469, A45470) for cells and tissue. This combination enables the isolation of longer DNA fragments, thus broadening the scope of your genomic research.

Specifications

Content And Storage • 0.15 mL MagMAX HMW DNA Booster, 100 preps
Format Reagent
Isolation Technology Magnetic Bead
Sample Type Fresh or Frozen Blood, Tissue, Cells
For Use With (Application) Long-read sequencing, and other molecular applications
No. of Reactions 100 Preps
Product Line MagMAX
Product Type DNA Booster
Quantity 0.15 μL
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

What KingFisher Purification System accessories are needed to automate the HMW DNA isolation workflow?

For the KingFisher Flex automated workflows and KingFisher Apex automated workflows, customers will require the 96 deep-well magnetic head and corresponding 96 deep well heat block. For the KingFisher Duo Prime automated workflows, customers will require the 12-pin magnet head for 96 deep-well plate formats and the corresponding KingFisher Duo Prime heat block
KingFisher Sample Purification System Accessories.

What will happen if the MagMAX HMW DNA Booster is not added during the workflow?

The isolated DNA recovered would likely not achieve greater than 100 kb in fragment size and the workflow reproducibility of the HMW kit would be compromised. If the customer wishes to leave the Booster Solution out of the workflow, we recommend following the guidance of Applied Biosystems(TM) MagMAX(TM) DNA Multi-Sample Ultra 2.0 kit to achieve genomic DNA without expanding into the HMW (>100 bp) or Ultra-HMW DNA (around 300 bp) recovery range.

Why is it recommended to use a mixture of wide bore and standard P1000 pipette tips for the MagMAX HMW DNA workflow?

Fragile samples can get damaged when they are forced through the narrow orifice of standard pipette tips. Using a combination of standard and wide bore pipette tips helps to increase the yield of HMW DNA, keeping the fragment sizes intact. Reference the MagMAX HMW DNA User Guide (MAN1001478).

Are low-binding tubes essential for manual workflows?

Yes, low-bind tubes are recommended to minimize loss due to absorption to the tube walls. Please refer to MagMAX HMW DNA User Guide (MAN1001478) for guidance on when to transfer to new tubes to reduce risk to sample contamination.

Why does the MagMAX HMW DNA User Guide specify a step-by-step reagent addition when preparing the sample plate for blood and cell samples, but recommends a master mix (pre-prepared mixture of reagents) for tissues?

The enhancer solution improves proteinase K activity at optimal concentrations; however, excessive enhancer solution can reduce proteinase K effectiveness. For blood and cell samples, adding the blood sample and cell lysate in the correct order dilutes the enhancer to achieve optimal proteinase K activity during the digestion step. For tissue samples, the tissue must be digested with proteinase K before using the tissue lysate for extraction. Preparing a master mix with Cell/Tissue extraction buffer ensures the enhancer solution is diluted to the concentration needed for optimal proteinase K activity in these samples.

Is it necessary to use all wells within the KingFisher 96 deep-well plate?

No, it is not necessary to fill all wells of a KingFisher 96 deep-well plate. If you are using less than the full-plate format, simply don't add any reagents to the unused wells for the isolation workflow.

Are there larger volume standalone reagent components available to scale up the MagMAX HMW DNA workflow?

Yes, the following larger volume standalone components are available for purchase separately:
- MagMAX HMW DNA Booster, 1,000 preps (1.0 mL) (Cat. No. A36625)
- MagMAX DNA Multi-Sample Ultra 2.0 Proteinase K (45 mL) (Cat. No. A36578)
- MagMAX DNA Multi-Sample Ultra 2.0 Binding Beads (45 mL) (Cat. No. A36579)
- MagMAX DNA Multi-Sample Ultra 2.0 Wash I Solution (1.1 L) (Cat. No. A36580)
- MagMAX DNA Multi-Sample Ultra 2.0 Lysis Binding Solution (450 mL) (Cat No. A36581)
- MagMAX DNA Multi-Sample Ultra 2.0 Elution Solution (120 mL) (Cat No. A36582)
- MagMAX DNA Multi-Sample Ultra 2.0 Enhancer Solution (45 mL) (Cat No. A36583)
- MagMAX Cell and Tissue DNA Extraction Buffer (600 mL) (Cat No. A45470)

Is RNase A treatment necessary when isolating HMW DNA from cells or tissue samples?

The RNase A treatment is optional but highly recommended for optimal results. RNAse A treatment helps to remove RNA and single stranded DNA, increasing the purity of double stranded DNA. We recommend using the PureLink RNase A (20 mg/mL) (Cat. No. 12091021).

Can I perform a manual and automated nucleic acid isolation using the same MagMAX HMW DNA Kit?

Yes, the MagMAX HMW DNA Kit has been optimized for manual nucleic acid isolation using the DynaMag-2 Magnet (Cat. No. 12321D) in addition to automated isolation using the KingFisher Duo Prime, Flex, or Apex Purification Systems.

If I have already purchased the MagMAX DNA Multi-Sample Ultra 2.0 Kit or standalone components, what do I need to extract high molecular weight DNA?

In addition to the MagMAX DNA Mult-Sample Ultra 2.0 product(s) you will need:
MagMAX HMW DNA Booster, 100 preps (Cat. No. A36624) or
In addition to the MagMAX DNA Mult-Sample Ultra 2.0 product(s) you will need:
MagMAX HMW DNA Booster, 100 preps (Cat. No. A36624) or MagMAX HMW DNA Booster, 1,000 preps (Cat No. A36625).

For cell and tissue samples include:
MagMAX Cell and Tissue DNA Extraction Buffer, 100 preps (Cat. No. A45469) or MagMAX Cell and Tissue DNA Extraction Buffer, 1,000 preps (Cat. No. A45470)
The MagMAX HMW DNA User Guide (MAN1001478)
The specific HMW DNA KingFisher automation protocol for your KingFisher Purification System (see product description page for download).
MagMAX HMW DNA Booster, 1,000 preps (Cat No. A36625).

For cell and tissue samples include:
MagMAX Cell and Tissue DNA Extraction Buffer, 100 preps (Cat. No. A45469) or MagMAX Cell and Tissue DNA Extraction Buffer, 1,000 preps (Cat. No. A45470)
The MagMAX HMW DNA User Guide (MAN1001478)
The specific HMW DNA KingFisher automation protocol for your KingFisher Purification System (see product description page for download).

What sample types and corresponding input amounts were developed with the MagMAX HMW DNA Kit workflow?

Whole blood: 200 µL, Cells: 1 million (1 x 106), Tissue: low DNA yield tissue (i.e., brain) 8 mg/ high DNA yield tissue (i.e., spleen) ~2 mg.
What is the expected fragment size profile achieved with the MagMAX HMW DNA Kit?

With the MagMAX HMW DNA Kit, you can expect greater than 70% of your DNA fragments to be above 40 kb, achieving a primary fragment peak larger than 100 kb. If a fragment size below 40 kb is desired, we recommend using the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570).

Can the MagMAX HMW DNA Booster be paired with other genomic DNA isolation kits?

The MagMAX HMW DNA Booster is specifically designed and optimized for use with the MagMAX DNA Multi-Sample Ultra 2.0 (Cat. No. A36570) chemistry.

What is included in the MagMAX HMW DNA Kit?

The MagMAX HMW DNA Kit (Cat. No. A36626) includes: MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570), MagMAX HMW DNA Booster, 100 preps (Cat. No. A36624).

MagMAX HMW DNA Kit with Cell and Tissue DNA Extraction Buffer (Cat. No. A26627) includes: MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570), MagMAX Cell and Tissue DNA Extraction Buffer (Cat. No. A45469), MagMAX HMW DNA Booster, 100 preps (Cat. No. A36624).

For Research Use Only. Not for use in diagnostic procedures.