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Applied Biosystems™ MagMAX™ DNA Multi-Sample Ultra 2.0 Kit

Catalog No. A36570
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Description

The MagMAX DNA Multi-Sample Ultra 2.0 Kit is an automatable magnetic bead-based sample preparation technology for the isolation of genomic DNA (gDNA) from 50 μL to 2 mL of whole blood, saliva, buffy coat, and buccal swabs.

The MagMAX DNA Multi-Sample Ultra 2.0 Kit is an automatable magnetic bead-based sample preparation technology for the isolation of genomic DNA (gDNA) from 50 μL to 2 mL of whole blood, saliva, buffy coat, and buccal swabs. The resulting purified gDNA is ideal for a variety of downstream molecular biology applications such as qPCR, next-generation sequencing, and microarray analysis. One kit is sufficient to process 200 50–200 μL samples or 100 200–400 μL samples except for buffy coat samples. For buffy coat only, one kit is sufficient to process 40–60 samples at 50–200 uL input volume. To process up to 200 buffy coat samples, please purchase additional Elution Solution (Cat. No. A36582).

Key features of the MagMAX DNA Multi-Sample Ultra 2.0 Kit:
• Process 6–96 samples in 45 minutes with 5 minutes hands-on time
• Isolate gDNA from 50 μL–2 mL of sample using a simple protocol in SBS microtiter plates
• Skip sample and buffer volume normalization steps for hassle-free processing
• Download validated BindIt software automation scripts for KingFisher Duo Prime and Flex purification systems
• Automation-friendly stand-alone MagMAX DNA Multi-Sample Ultra 2.0 reagent bottle options available

Isolate genomic DNA from multiple sample types with a single sample prep kit
The MagMAX DNA Multi-Sample Ultra 2.0 Kit isolates gDNA from 50 μL–2 mL of whole blood (figure below), fresh and stabilized saliva (figure below), and EDTA/Streck cfDNA tube buffy coat (figure below) samples. Additionally, the same kit can be used to isolate gDNA from buccal swabs and other biological sample types. The MagMAX DNA Multi-Sample Ultra 2.0 Kit maximizes gDNA yields by treating samples with proteinase K (PK) and PK-enhancer followed by an innovative lysis/binding solution to simultaneously lyse cells, denature proteins, and capture gDNA on the high-binding capacity Ultra 2.0 gDNA magnetic binding beads. The Ultra 2.0 Wash I solution removes proteins, detergents, and other impurities from the bound nucleic acid. The gDNA is then released from the beads using a low-TE elution solution.

Fast and hassle-free MagMAX magnetic bead-based gDNA workflows
The MagMAX DNA Multi-Sample Ultra 2.0 Kit combines multiple laborious sample processing steps together into a single step to minimize the time and effort traditionally required by other methods without compromising yield and purity. Process a broad range of sample volume inputs simultaneously without sample normalization or reagent volume adjustments for hassle-free sample prep. Simply match your sample volumes to one of three standard input workflows (50 μL to 400 μL, up to 1 mL, up to 2 mL) and follow the work instructions for either manual or semi-automated sample preparation.

Made for KingFisher purification systems
The MagMAX DNA Multi-Sample Ultra 2.0 Kit is optimized for use on the moderate-throughput KingFisher Duo Prime (Cat. No. 5400110) and the higher-throughput KingFisher Flex (Cat. No. 5400630) purification systems. Users can process up to 400 μL of sample volume in high-throughput SBS 96-well deep-well microtiter plates using the Flex system. For smaller sample batches, users can process 12 samples using the Duo Prime system. Larger sample input volumes (1–2 mL), which are more challenging to process, are purified in SBS 24-well deep-well microtiter plates for either 24 samples on the Flex or 6 samples on the Duo Prime system. KingFisher instrument BindIt software scripts for all sample types and both Flex and Duo Prime systems can be downloaded below and installed onto the KingFisher instruments.

Working with higher throughput sample numbers?
The same MagMAX DNA Multi-Sample Ultra 2.0 Kit reagents are available as automation-friendly stand-alone reagents. MagMAX DNA Multi-Sample Ultra 2.0 stand-alone reagents are individually bottled in larger reagent volumes to make your high-thoughput workflows easier to handle. When purchased together as a set of six stand-alone reagents, users will have sufficient volume of MagMAX DNA Multi-Sample Ultra 2.0 reagents to process 2000 50–200 uL samples or 1000 200–400 uL samples.

MagMAX DNA Ultra Multi-Sample 2.0 Proteinase K, 45 mL (Cat. No. A36578)
MagMAX DNA Ultra Multi-Sample 2.0 Binding Beads, 45 mL (Cat. No. A36579)
MagMAX DNA Ultra Multi-Sample 2.0 Wash I Solution, 1.1 L (Cat. No. A36580)
MagMAX DNA Ultra Multi-Sample 2.0 Lysis/Binding Solution, 450 mL (Cat. No. A36581)
MagMAX DNA Ultra Multi-Sample 2.0 Elution Solution, 120 mL (Cat. No. A36582)
MagMAX DNA Ultra Multi-Sample 2.0 Enhancer Solution, 45 mL (Cat. No. A36583)

Specifications

Content And Storage • 4.5 mL MagMAX DNA Multi-Sample Ultra 2.0 Proteinase K, store at room temperature
• 4.5 mL MagMAX DNA Multi-Sample Ultra 2.0 Binding Beads, store at room temperature
• 110 mL MagMAX DNA Multi-Sample Ultra 2.0 Wash I Solution, store at room temperature
• 45 mL MagMAX DNA Multi-Sample Ultra 2.0 Lysis/Binding Solution, store at room temperature
• 12 mL MagMAX DNA Multi-Sample Ultra 2.0 Elution Solution, store at room temperature
• 4.5 mL MagMAX DNA Multi-Sample Ultra 2.0 Enhancer Solution, store at room temperature
Format Kit
For Use With (Application) Sequencing
Product Line MagMAX
Product Type DNA Multi-Sample Ultra 2.0 Kit
Quantity 1 Kit
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

I want to isolate genomic DNA from buccal cells. How do I do this?

We would recommend using one of our MagMAX DNA Multi-Sample Ultra 2.0 Kit or ChargeSwitch gDNA Buccal Cell Kits, which were designed to isolate high-quality genomic DNA from buccal samples. We also offer normalized kits, which will normalize isolated DNA concentration from sample to sample.
What will happen if the MagMAX HMW DNA Booster is not added during the workflow?

The isolated DNA recovered would likely not achieve greater than 100 kb in fragment size and the workflow reproducibility of the HMW kit would be compromised. If the customer wishes to leave the Booster Solution out of the workflow, we recommend following the guidance of Applied Biosystems(TM) MagMAX(TM) DNA Multi-Sample Ultra 2.0 kit to achieve genomic DNA without expanding into the HMW (>100 bp) or Ultra-HMW DNA (around 300 bp) recovery range.

If I have already purchased the MagMAX DNA Multi-Sample Ultra 2.0 Kit or standalone components, what do I need to extract high molecular weight DNA?

In addition to the MagMAX DNA Mult-Sample Ultra 2.0 product(s) you will need:
MagMAX HMW DNA Booster, 100 preps (Cat. No. A36624) or
In addition to the MagMAX DNA Mult-Sample Ultra 2.0 product(s) you will need:
MagMAX HMW DNA Booster, 100 preps (Cat. No. A36624) or MagMAX HMW DNA Booster, 1,000 preps (Cat No. A36625).

For cell and tissue samples include:
MagMAX Cell and Tissue DNA Extraction Buffer, 100 preps (Cat. No. A45469) or MagMAX Cell and Tissue DNA Extraction Buffer, 1,000 preps (Cat. No. A45470)
The MagMAX HMW DNA User Guide (MAN1001478)
The specific HMW DNA KingFisher automation protocol for your KingFisher Purification System (see product description page for download).
MagMAX HMW DNA Booster, 1,000 preps (Cat No. A36625).

For cell and tissue samples include:
MagMAX Cell and Tissue DNA Extraction Buffer, 100 preps (Cat. No. A45469) or MagMAX Cell and Tissue DNA Extraction Buffer, 1,000 preps (Cat. No. A45470)
The MagMAX HMW DNA User Guide (MAN1001478)
The specific HMW DNA KingFisher automation protocol for your KingFisher Purification System (see product description page for download).

Can the MagMAX HMW DNA Booster be paired with other genomic DNA isolation kits?

The MagMAX HMW DNA Booster is specifically designed and optimized for use with the MagMAX DNA Multi-Sample Ultra 2.0 (Cat. No. A36570) chemistry.

What is included in the MagMAX HMW DNA Kit?

The MagMAX HMW DNA Kit (Cat. No. A36626) includes: MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570), MagMAX HMW DNA Booster, 100 preps (Cat. No. A36624).

MagMAX HMW DNA Kit with Cell and Tissue DNA Extraction Buffer (Cat. No. A26627) includes: MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570), MagMAX Cell and Tissue DNA Extraction Buffer (Cat. No. A45469), MagMAX HMW DNA Booster, 100 preps (Cat. No. A36624).

What DNA extraction kit would you recommend for fragments smaller than 250 kb that are compatible with a KingFisher Flex Purification System?

We recommend the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570) that is optimized for use on the moderate-throughput KingFisher Duo Prime Purification System (Cat. No. 5400110) and the higher-throughput KingFisher Flex Purification System, KingFisher with 96 Deep-well head (Cat. No. 5400630).
What is the difference between Proteinase K for MagMAX DNA Multi-Sample Ultra Kit (Cat. No A25561) and Proteinase K for MagMAX 96 DNA Multi-Sample Kit (Cat. No. 4489110)?

The difference between Proteinase K for MagMAX‐96 DNA Multi‐Sample Kit and Proteinase K for MagMAX DNA Multi‐Sample Ultra Kit is the brand. The former is from Invitrogen legacy and the latter is from Applied Biosystems legacy. Both products have an identical concentration of 100 mg/mL and can be used interchangeably.
Are the DNA Binding Beads included within the MagMAX DNA Multi-Sample Ultra 2.0 Binding Beads (Cat. No. A36570) available as a stand-alone product and in a smaller volume?

We offer the MagMAX DNA Multi-Sample Ultra 2.0 Binding Beads (Cat. No. A36579), but the volume is 45 mL. The volume of the beads in the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570) is 4.5 mL.
What is the level of detection for somatic variants with the Oncomine Myeloid Research Assay GX?

Detection of somatic variants with the Oncomine Myeloid Research Assay GX has been verified down to 5% allele frequency.
Which extraction kits are compatible with Oncomine Myeloid Research Assay GX?

Here is a list of kits that are compatible with Oncomine Myeloid Research Assay GX:

Nucleic acid isolation - RNA samples
- MagMAX mirVana Total RNA Isolation Kit, Cat. No. A27828
- PureLink Total RNA Blood Kit, Cat. No. K156001
- RNaseZap RNase Decontamination Solution, Cat. No. AM9780, AM9782, AM9784

Nucleic acid isolation - DNA samples
- MagMAX DNA Multi-Sample Ultra 2.0 Kit, Cat. No. A36570
- PureLink Genomic DNA Mini Kit, Cat. No. K1820-01
What are the sample input requirements for Oncomine Myeloid Research Assay GX?

The recommended minimum sample input is 27.75 ng of DNA (1.11 ng/µL minimum concentration) and 14.25 ng of RNA (0.95 ng/µL minimum concentration).
What sample controls should I use with the Oncomine Myeloid Research Assay GX?

The recommended controls are:

DNA Controls:
- Seraseq® Myeloid Mutation DNA Mix, Cat. No. 0710-0408 (SeraCare®)
- Myeloid DNA Reference Standard, Cat. No. HD829 (Horizon™)
- AcroMetrix™ Oncology Hotspot Control, Cat. No. 969056

RNA Controls:
- Universal Human Reference RNA, Cat. No. 740000 (Agilent™)
- Seraseq® Myeloid Fusion RNA Mix, Cat. No. 0710-0407 (SeraCare®)
How many Oncomine Myeloid Research Assay GX samples can be multiplexed on a GX5 Chip?

Multiplex sequencing of up to 8 Oncomine Myeloid Research Assay GX samples (8 DNA+ 8 RNA) per lane on a GX5 Chip can be performed in a single run. There are 4 lanes on the GX5 Chip, so 32 total samples (32 DNA+ 32 RNA) can be run on one GX5 Chip (8 samples per run).

What are the components of Oncomine Myeloid Research Assay GX?

The Oncomine Myeloid Research Assay GX (Cat. No. A47857) includes the 2-pool DNA panel, the 1-pool RNA panel, Genexus Strip 1, and Genexus Strip 2-AS. The DNA panel is provided in two packs of 8 tubes (4 tubes of Myeloid DNA Pool 1 and 4 tubes of Myeloid DNA Pool 2 per pack). The RNA panel is provided in one pack of 8 tubes (Myeloid RNA Pool 1). Each primer pool in the panel is provided in pairs of tubes, where each tube pair contains one tube with primers in position 1 and one empty uncapped tube in position 2. Three 8-strip packs of the Genexus Strip 1 and Genexus Strip 2-AS (24 total of each strip) are provided with each kit.
How many samples can I run using Oncomine Myeloid Research Assay GX?

The contents of each Oncomine Myeloid Research Assay GX kit are sufficient for up to 32 samples (32 x 3‑pool reactions: 32 x 2‑pool DNA reactions and 32 x 1‑pool RNA reactions).
How many primer pools are present in the Oncomine Myeloid Research Assay GX?

The Oncomine Myeloid Research Assay GX includes 3 pools of Ion AmpliSeq oligonucleotide primers (a 2-pool DNA panel and a 1-pool RNA panel).
What is the Oncomine Myeloid Research Assay GX gene content?

The Oncomine Myeloid Research Assay GX targets key genes and fusions associated with major myeloid disorders, including acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML). The DNA panel comprises 40 key genes while the RNA panel includes a broad fusion panel of 29 driver genes, enabling detection over 600 unique fusion isotypes. See flyer for gene targets (https://assets.thermofisher.com/TFS-Assets/CSD/Flyers/Oncomine_myeloid_assay_gx_flyer.pdf).

What is the Oncomine Myeloid Research Assay GX designed for ?

The Oncomine Myeloid Research Assay GX is a comprehensive targeted next-generation sequencing (NGS) assay designed for sensitive detection of myeloid disorder-associated DNA mutations and fusion transcripts in blood and bone marrow samples.
Can the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570) be used to isolate genomic DNA from bone marrow aspirates and PBMCs?

Yes, we have protocols for using the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570) to isolate genomic DNA from these sample types, but they require purchase of an additional Cell and Tissue Extraction Buffer. The kit and buffer can be purchased together as Cat. No. A45721 or the Cell and Tissue Extraction Buffer can be purchased as a stand alone item (Cat. No. A45469 or A45470).

The suggested workflow for bone marrow aspirates is as follows:
- Use only up to 200 µL of bone marrow aspirate
- Centrifuge at 200 x g for 10 mins
- Remove the supernatant (leaving about 50 µL of aspirate with the loose pellet) and resuspend the cell pellet in 400 µL of Cell and Tissue Extraction Buffer
- From this point, follow the protocol for isolation of DNA from buffy coat samples

The suggested workflow for 1-2.0 x 10E6 PBMCs is as follows:
- Centrifuge at 500-800 x g for 10 mins
- Remove the supernatant (leaving the cell pellet) an resuspend the cell pellet in 400 L of Cell and Tissue Extraction Buffer
- From this point, follow the protocol for isolation of DNA from cultured cell samples
Can the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570) be used to isolate DNA from cultured cells and tissue?

Yes, we have protocols for using the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570) to isolate DNA from cells and tissue, but they require purchase of an additional Cell and Tissue Extraction Buffer. The kit and buffer can be purchased together as Cat. No. A45721 or the Cell and Tissue Extraction Buffer can be purchased as a stand alone item (Cat. No. A45469 or A45470).

Do the MagMAX DNA Multi-Sample Ultra 2.0 cell and tissue DNA isolation protocols require an RNase A treatment step?

RNase A digestion is optional, but we highly recommend doing it. We recommend using PureLink RNase A (20 mg/mL) (Cat. No. 12091021).
Saliva samples can be processed with the MagMAX DNA Multi-Sample Ultra 2.0 Kit as well as MagMAX Saliva gDNA Isolation Kit. What is the difference between these kits and which one should I use?

Both kits use different magnetic beads and different buffers. Both kits can be used with stabilized and fresh saliva samples, but if you are exclusively working with saliva samples, we recommend using the MagMAX Saliva gDNA Isolation Kit. This kit has a faster and more optimized workflow for isolating genomic DNA from saliva samples.

For Research Use Only. Not for use in diagnostic procedures.