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  5. Thermo Scientific™ Lambda Exonuclease (10 U/μL)

Thermo Scientific™ Lambda Exonuclease (10 U/μL)

Catalog No. FEREN0561
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1,000 units
5,000 units
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FEREN0561 1,000 units
FEREN0562 5,000 units
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Catalog No. FEREN0561 Supplier Thermo Scientific™ Supplier No. EN0561

Description

Lambda Exonuclease is a highly processive exodeoxyribonuclease that selectively digests the 5'-phosphorylated strand of double-stranded DNA in 5'-3' direction.

Thermo Scientific Lambda Exonuclease is a highly processive 5'≥3' exodeoxyribonuclease. It selectively digests the 5'-phosphorylated strand of double-stranded DNA. The enzyme exhibits low activity on single-stranded DNA and non-phosphorylated DNA, and has no activity at nicks and limited activity at gaps in DNA.

Highlights

  • Active in Thermo Scientific PCR buffers

Applications

  • Generating single-stranded PCR products for use in:
    • DNA sequencing
    • Analysis of DNA single-strand conformation polymorphism (SSCP)
    • Rolling circle amplification
     
  • Producing single-stranded DNA from double-stranded DNA fragments
  • Cloning of PCR products

Note

Use of this enzyme in certain applications may be covered by patents and may require a license.

Specifications

Concentration 10 U/μL
Enzyme Exonuclease
Quantity 1,000 units
Product Type Exonuclease

For Research Use Only. Not for use in diagnostic procedures.