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Thermo Scientific™ Klenow Fragment, exo-

Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.

$123.90 - $489.30

Specifications

Concentration 5U/µL
Inhibitors metal chelators, PPi, Pi (at high concentrations) (7)
Molecular Weight 68kDa monomer
Source E.coli cells with a cloned DNA fragment of the mutated polA gene
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Description & Specifications

Specifications

Concentration 5U/µL
Inhibitors metal chelators, PPi, Pi (at high concentrations) (7)
Molecular Weight 68kDa monomer
Source E.coli cells with a cloned DNA fragment of the mutated polA gene

Klenow Fragment, exo-, is the large fragment of DNA polymerase I . It exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities of DNA Polymerase I. The 3'→5' exonuclease activity of the enzyme is eliminated by mutations in the 3'→5'-exonuclease active sit.

Highlights

  • Lacks 3'→5' exonuclease activity
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, fluorescein-, rhodamine-, aminoallyl-, biotin-labeled nucleotides)
  • Active in restriction enzyme, PCR, and RT buffers