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Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)

Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.

$241.60 - $918.60

Specifications

Concentration 5 U/μL
Polymerase DNA Polymerase I
Compatible Buffer Restriction Enzyme, PCR, RT Buffer
Product Type Klenow Fragment
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Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
FEREP0421
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Thermo Scientific™
EP0421
300 units
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FEREP0422
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Thermo Scientific™
EP0422
1,500 units
Each for $918.60
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Description

Description

Klenow Fragment, exo-, is the large fragment of DNA polymerase I . It exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities of DNA Polymerase I. The 3'→5' exonuclease activity of the enzyme is eliminated by mutations in the 3'→5'-exonuclease active sit.

Highlights

  • Lacks 3'→5' exonuclease activity
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, fluorescein-, rhodamine-, aminoallyl-, biotin-labeled nucleotides)
  • Active in restriction enzyme, PCR, and RT buffers
Specifications

Specifications

5 U/μL
DNA Polymerase I
Restriction Enzyme, PCR, RT Buffer
Klenow Fragment
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For Research Use Only. Not for use in diagnostic procedures.