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Promega Klenow Fragment, DNA Polymerase I

Catalog No. PRM2201
Encompass
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Quantity:
150 U
500 U
Concentration:
5 to 10 U/μL
5 U/μL
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Catalog No. Quantity Concentration
PRM2201 150 U 5 U/μL
PRM2206 500 U 5 to 10 U/μL
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Catalog No. PRM2201 Supplier Promega Supplier No. M2201

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

A DNA-dependent DNA polymerase that lacks the 5'→3' exonuclease activity of intact E. coli DNA Polymerase I but retains its 5'→3' polymerase, 3'→5' exonuclease and strand displacement activities.

  • DNA-Dependent DNA Polymerase that Lacks the 5′→3′ Exonuclease Activity
  • Use in a variety of molecular applications
  • Heat-inactivate by heating at 75°C for 10 minutes
  • Active in many Promega 1X restriction enzyme buffers
  • Supplied at a concentration of 5u/μL

†DNA polymerase kit that includes 5μmoles each of dATP, dCTP, dGTP, and dTTP at 10mM in water.

Specifications

Concentration 5 U/μL
Color Colorless
Components 10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT
Purity >80%
pH 7.2
For Use With (Application) Filling-in or labeling 3' ends of 5' overhanging double-stranded DNA, second-strand cDNA synthesis, DNA sequencing by the Dideoxy method
Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Content And Storage -30°C to -10°C
Source Recombinant E. coli strain
Quantity 150 U
Product Type DNA Polymerase I Large (Klenow) Fragment
Molecular Weight (g/mol) 68
Form Liquid
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