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Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning 
Combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO vector to allow easy, high-efficiency cloning of blunt-end PCR products
Supplier: Invitrogen™ K283020
Includes: Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid
Description
- Unique technology to minimize background
- Get your clone: 95% efficiency
- Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient
- Kanamycin and Zeocin resistance genes for your choice of selection
- Convenient, validated restriction sites in MCS for further subcloning
- Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time
Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Cloning, Methylation Analysis, PCR Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Sequencing Bisulfite-Treated DNA
Specifications
| Mach1™ | |
| Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
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| Chromatin Biology | |
| PCR Cloning Kit | |
| Blunt DNA Cloning Vectors |
| Blunt TOPO™ | |
| Kit | |
| One Shot | |
| 25 Reactions |
For Research Use Only. Not for use in diagnostic procedures.