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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli DFS Item

Offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases

$944.00 - $1,626.00

Specifications

Bacterial/Yeast Strain DH5α™
Product Type Kit, PCR Cloning
For Use With (Application) Cloning, PCR Cloning
Includes Box 1: Linearized and topoisomerase I-activated pCRBlunt II-TOPO vector, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes: Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid


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Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
K282040 Invitrogen™
LSK282040
50 reactions Each for $1,626.00
K282020 Invitrogen™
LSK282020
25 reactions Each for $944.00
Specifications

Description

Kit includes linearized and topoisomerase I-activated pCR™-Blunt II-TOPO Vector for 5-minute benchtop ligations without ligase.

Selected Zero Blunt TOPO PCR Cloning Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO-cloned inserts for downstream analysis.

  • ccdB gene for positive selection
  • EcoR I sites flanking the PCR product insertion site for easy excision of inserts
  • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli
  • SP6 promoter/primer site for in vitro RNA transcription and sequencing
  • M13 forward and reverse primer sites for sequencing or PCR screening

Applications:

Cloning, PCR Cloning

Description & Specifications

Specifications

DH5α™
Kit, PCR Cloning
Cloning, PCR Cloning
Box 1: Linearized and topoisomerase I-activated pCRBlunt II-TOPO vector, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid