Please login to your online account to display your discounted pricing

Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells DFS Item

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

$435.75 - $724.50

Specifications

Product Type Kit, PCR Cloning
For Use With (Application) Cloning, PCR Cloning
Includes Zero Blunt PCR cloning kits contain linearized pCR-Blunt vector, ExpressLink T4 DNA ligase , 5X ExpressLink T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers
Quantity 20 reactions
View More Specs
Includes:  Zero Blunt™ PCR cloning kits contains: linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

K275020

DFS Item
 
invitrogen™
K275020
20 reactions Each for $435.75

K275040

DFS Item
 
invitrogen™
K275040
40 reactions Each for $724.50
Description & Specifications

Specifications

Product Type Kit, PCR Cloning
For Use With (Application) Cloning, PCR Cloning
Includes Zero Blunt PCR cloning kits contain linearized pCR-Blunt vector, ExpressLink T4 DNA ligase , 5X ExpressLink T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers
Quantity 20 reactions

The Zero Blunt™ PCR Cloning Kit uses the multipurpose cloning vector pCR™-Blunt and ExpressLink™ T4 DNA Ligase to generate a ligation product in a five-minute, room-temperature ligation step.
The pCR-Blunt vector provides:

  • EcoR I sites flanking the PCR product insertion site for excision of inserts
  • T7 promoter/primer site for in vitro RNA transcription and sequencing
  • M13 forward and reverse primer sites for sequencing or PCR screening
How Zero Blunt PCR Cloning Works
The Zero Blunt PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants. The pCR-Blunt vector contains the lethal E. coliccdB gene fused to the C-terminus of LacZα (Bernard et al., 1994). Ligation of a blunt PCR fragment disrupts expression of the lacZα-ccdB gene fusion permitting growth of only positive recombinants upon transformation. Cells that contain non-recombinant vector are killed when the transformation mixture is plated.
Kit Configurations
The Zero Blunt PCR Cloning Kit is offered in a variety of configurations: with One Shot™ TOPO10 Chemically Competent E. coli (K2700-20 and K2700-40) and without competent cells (K2750-20 and K2750-40) in 20- and 40- reaction kit sizes.

  • Fast and convenient: 5-minute, room-temperature ligation
  • Efficient: ccdB gene for positive selection results in >80% clones with correct insert
  • Flexible: choice of kanamycin or Zeocin™ resistance for flexible antibiotic selection

Cloning, PCR Cloning

Shipping Condition: Dry Ice