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Invitrogen™ X-Gal

Catalog No. 15520034
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Quantity:
100 mg
1 g
Product Type:
X-Gal Substrate
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Catalog No. Quantity Product Type
15520034 100 mg X-Gal Substrate
15520018 1 g X-Gal Substrate
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Catalog No. 15520034 Supplier Invitrogen™ Supplier No. 15520034
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Description

X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays.

X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays. Bacteria containing active β-galactosidase produce blue colonies when grown on plates containing X-Gal. X-Gal is supplied as white crystals and is soluble in dimethylformamide.

Primary applications

  • Blue-white screening—detect functional β-galactosidase (reporter gene expression)
  • Histochemical detection of β-galactosidase activity in fixed tissues
  • X-gal overlay assay

Find the media format that fits your needs

We offer many ready-to-use media formulations, such as LB Broth (10855001), Terrific Broth (A1374301) or S.O.C recovery medium (15544034).

We also offer IPTG (15529019) for blue-white screening.

Explore other media formats for bacterial growth.

Order Info

Approved for shipment at Room Temperature or on Wet Ice.

Specifications

Chemical Name or Material 5-bromo-4-chloro-3-indolyl-β-D-galactoside
Content And Storage Store in freezer (-5°C to -30°C).
Detection Method Colorimetric
Description X-Gal, Cell-Permeant
Format Bottle
For Use With (Application) Immunocytochemical Assays
Product Type X-Gal Substrate
Quantity 100 mg
Shipping Condition Approved for shipment at Room Temperature or on Wet Ice

For Research Use Only. Not for use in diagnostic procedures.