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Invitrogen™ UltraPure™ Salmon Sperm DNA Solution

Catalog No. 15632011
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15632011 5 x 1 mL
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Catalog No. 15632011 Supplier Invitrogen™ Supplier No. 15632011
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Description

Includes

5 tubes Salmon Sperm DNA solution (1mL each, 10mg/mL)

Used directly in preparation of prehybridization and hybridization solutions

UltraPure™ Salmon Sperm DNA Solution is a ready-to-use, sheared DNA solution that is used directly in the preparation of prehybridization and hybridization solutions.

  • Prepared from highly pure, phenol/chloroform-extracted DNA and DNase-free, RNase-free (DEPC-treated), distilled, deionized water
  • DNA is sheared to average size of ≤2,000 bp
  • Concentration is adjusted to 10 mg/mL
  • Used to block nonspecific attachment of probe to surface of membrane

ChIP-on-Chip, Chromatin Biology, DNA & RNA Purification & Analysis, Gene Expression Analysis & Genotyping, Microarray Analysis, Northern Blotting, Nucleic Acid Gel Electrophoresis & Blotting, RNAi, Epigenetics & Non-Coding RNA Research, Southern Blotting

Order Info

Shipping Conditions: Dry Ice

Specifications

Content And Storage 5 tubes Salmon Sperm DNA solution (1 ml each, 10 mg/ml)

UltraPure™ Salmon Sperm DNA is stable for 1 year at -20°C.
For Use With (Application) Chromatin Biology, Nucleic Acid Gel Electrophoresis, Blotting
Key Functions Block Non-Specific Hybridization
Product Line UltraPure
Product Type Salmon Sperm DNA Solution
Quantity 5 x 1 mL
Reagent Type Salmon Sperm DNA
Shipping Condition Dry Ice

Frequently Asked Questions (FAQs)

What species of salmon is Ultrapure Salmon Sperm DNA derived from?

The species of salmon is Oncerhyncus keta.
Is Ultrapure Salmon Sperm DNA double stranded? How can I make it single stranded?

Ultrapure Salmon Sperm DNA is double stranded. It can be made single stranded by heating to 95 degrees C and then cooling rapidly on ice. Cooling is usually done with the DNA diluted in a hybridization solution which keeps it single stranded because of the large dilution volume.
What concentration should the UltraPure Salmon Sperm DNA Solution (Cat. No. 15632011) be used at?

Carrier DNA is typically used at a concentration of 100 µg/mL in both the prehybridization and hybridization solutions.

For Research Use Only. Not for use in diagnostic procedures.