missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. Miscellaneous Molecular Biology Reagents
  5. 15508013

Invitrogen™ UltraPure™ Dithiothreitol

Catalog No. 15508013
Encompass
Change view
Click to view available options
Quantity:
5 g
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
15508013 5 g
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. 15508013 Supplier Invitrogen™ Supplier No. 15508013

Description

UltraPure Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymes containing free sulfhydryl groups.

UltraPure Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymes containing free sulfhydryl groups. Higher concentrations of DTT are used to cleave disulfide linkages in polypeptides and to facilitate protein denaturation by detergents or chaotropic agents.

Order Info

Approved for shipment at Room Temperature or on Wet Ice

Specifications

Content And Storage Store in freezer (-5° to -30°C).
For Use With (Application) Protein Electrophoresis & Western Blotting
Product Line UltraPure
Quantity 5 g
Shipping Condition Approved for shipment at Room Temperature or on Wet Ice

Frequently Asked Questions (FAQs)

Can beta-mercaptoethanol (BME) be used rather than DTT as the reducing agent in the NuPAGE LDS Sample Buffer?

Either BME or DTT can be used in the NuPAGE LDS Sample Buffer.

Make sure that a fresh solution of BME is used. FINAL concentration:

DTT 50-100 mM

BME 2-5%
How do I prepare a 1 M DTT solution from powder and how should I store the solution?

Preparing 1 M DTT:
1) Dissolve 3.09 g DTT in 20 mL distilled H2O.
2) Dispense into 1 mL aliquots.
3) Store the aliquots at -20 degrees C.
Note: Upon thawing the vial, there may be some precipitation that can be re-dissolved by warming and mixing.

For Research Use Only. Not for use in diagnostic procedures.